PMID: 7526162Nov 1, 1994Paper

Spontaneous mutation at codon 61 of the Ha-ras gene in the nascent liver of B6C3F1, C3H/He and C57BL/6 mice

Mutation Research
B A Moulds, Jay I Goodman

Abstract

DNA was isolated from the liver of young B6C3F1, C3H/He and C57BL/6 mice, 6-9 weeks old. A portion of exon 2 of Ha-ras was amplified by PCR allele-specific amplification. The PCR product was identified by (a) size, (b) presence of a diagnostic restriction site, and (c) direct sequencing. Our results indicate that nascent mouse liver bears a subpopulation of cells which contain a mutation in codon 61 of Ha-ras, specifically an A to G transition at position 2. Therefore, the detection of this mutation in chemically induced mouse liver tumors does not demonstrate that the chemical in question acts as a mutagen. It might act by a nongenotoxic mechanism, i.e., by facilitating a clonal expression of cells bearing this spontaneous mutation.

References

Jan 1, 1992·Molecular Carcinogenesis·B L Schneider, G T Bowden
Nov 1, 1991·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·J I GoodmanT R Fox
Jan 1, 1991·Progress in Histochemistry and Cytochemistry·M SchwarzJ Mahr
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A BuchmannM Schwarz
Jan 1, 1989·Molecular Carcinogenesis·P Brookes
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R W WisemanJ A Miller

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