PMID: 7526175Nov 1, 1994Paper

Spontaneous mutations in lacI-containing lambda lysogens derived from transgenic mice: the observed patterns differ in liver and spleen

Mutation Research
A KnöllS S Sommer

Abstract

The pattern of somatic mutation observed in tumor suppressor genes, such as the p53 gene, vary dramatically with tumor type. Some of the observed differences are due to tissue specific effects of mutagens, but it is also possible that some differences may reflect the tissue/cell type specificity of spontaneous mutation. Transgenic mouse models with recombinant shuttle vectors containing the lacI or lacZ target genes may shed light on the extent to which spontaneous mutation displays tissue specificity. Herein we utilize a recently described selectable system to obtain spontaneous mutants for analysis of the molecular lesions. Spontaneous mutations were isolated in the lacI gene recovered from five transgenic mice carrying a lambda shuttle vector. Seventy-three and 67 independent mutations derived from liver and spleen DNA, respectively, were defined in the amino terminal region of lacI. Although technical barriers preclude a direct assessment of the E. coli derived pattern of mutation in this system, five pieces of circumstantial evidence suggest that many of the mutations arose in mouse rather than in E. coli. In DNA from both liver and spleen, mutations at CpG dinucleotides predominate (58% and 51%, respectively). In spleen, ...Continue Reading

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Citations

Jul 16, 1999·Teratogenesis, Carcinogenesis, and Mutagenesis·G J JenkinsJ M Parry
Sep 28, 2000·Mutation Research·P W HeinmöllerS S Sommer
Dec 31, 1997·Mutation Research·A AidooD A Casciano
Jan 1, 1996·Environmental and Molecular Mutagenesis·E G LeachP M Glazer
Jan 1, 1996·Environmental and Molecular Mutagenesis·H NishinoS S Sommer
Jun 3, 2000·Japanese Journal of Cancer Research : Gann·Y ShioyamaM Katsuki
Jan 29, 2000·Chemical Research in Toxicology·J R CarmicalR S Lloyd
Oct 17, 1998·Methods : a Companion to Methods in Enzymology·S E DePrimoJ R Stringer

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