PMID: 10601300Dec 22, 1999

Spontaneous neutrophil apoptosis involves caspase 3-mediated activation of protein kinase C-delta

The Journal of Biological Chemistry
J PongraczJanet M Lord


Neutrophils are short-lived leukocytes that die by apoptosis. Whereas stress-induced apoptosis is mediated by the p38 mitogen-activated protein (MAP) kinase pathway (Frasch, S. C., Nick, J. A., Fadok, V. A., Bratton, D. L., Worthen, G. S., and Henson, P. M. (1998) J. Biol. Chem. 273, 8389-8397), signals regulating spontaneous neutrophil apoptosis have not been fully determined. In this study we found increased activation of protein kinase C (PKC)-beta and -delta in neutrophils undergoing spontaneous apoptosis, but we show that only activation of PKC-delta was directly involved in the induction of apoptosis. PKC-delta can be proteolytically activated by caspase 3. We detected the 40-kDa caspase-generated fragment of PKC-delta in apoptotic neutrophils and showed that the caspase 3 inhibitor Asp-Glu-Val-Asp-fluoromethylketone prevented generation of the 40-kDa PKC-delta fragment and delayed neutrophil apoptosis. In a cell-free system, removal of PKC-delta by immunoprecipitation reduced DNA fragmentation, whereas loss of PKC-alpha, -beta, or -zeta had no significant effect. Rottlerin and LY379196 inhibit PKC-delta and PKC-beta, respectively. Only Rottlerin was able to delay neutrophil apoptosis. Inhibitors of MAP-ERK kinase 1 (PD98...Continue Reading


Jan 1, 1992·Journal of Leukocyte Biology·J I Smallwood, S E Malawista
Feb 9, 1989·The New England Journal of Medicine·S J Weiss
Nov 15, 1988·Analytical Biochemistry·C M BunceG Brown
Oct 1, 1987·Physiological Reviews·J C Hogg
Jun 1, 1995·Immunology Today·M B Hallett, D Lloyds
Feb 28, 1994·Biochemical and Biophysical Research Communications·M GschwendtF Marks
Jan 1, 1996·British Journal of Haematology·R E StringerS W Edwards
May 24, 1996·Biochemical and Biophysical Research Communications·G GriffithsJanet M Lord
May 31, 1996·The Journal of Biological Chemistry·J Y LeeL M Obeid
Jun 1, 1997·Molecular Pathology : MP·E DeaconJanet M Lord
May 20, 1998·Oncogene·R D Whelan, P J Parker
Oct 17, 1998·Molecular and Cellular Biology·A BhartiS Kharbanda
Apr 10, 1999·The Journal of Biological Chemistry·S FrutosM T Diaz-Meco
Aug 24, 1999·European Journal of Immunology·Dagmar Scheel-ToellnerJanet M Lord


Sep 5, 2002·Journal of Cellular Biochemistry·Jin-Shan ChenSeu-Mei Wang
Feb 15, 2001·Journal of Biomedical Science·B TabakoffP L Hoffman
Nov 13, 2003·Peptides·Ronald D MathisonEssam Metwally
Oct 29, 2003·Journal of Immunological Methods·Taisuke OhiraJohn A Badwey
Aug 21, 2001·Mechanisms of Ageing and Development·Janet M LordMike Salmon
Feb 11, 2003·Pharmacology & Therapeutics·Angela ClerkDonna M Valks
Aug 21, 2003·Free Radical Biology & Medicine·Cinzia DomenicottiUmberto Maria Marinari
Jul 3, 2003·Molecular and Cellular Neurosciences·E K ChoiW Wasco
Jun 5, 2003·International Immunopharmacology·Min Jung LeeJong Young Kwak
Aug 30, 2003·International Immunopharmacology·Jun HurJong Young Kwak
Apr 14, 2009·The Journal of Biological Chemistry·Paul J HurdTony Kouzarides
May 9, 2008·The Journal of Infectious Diseases·Qingming XiongYasuko Rikihisa
Aug 30, 2003·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Joanna H WebbBjörn Dahlbäck
Jan 16, 2007·Transplantation·Young Sam KwonJonathan F McAnulty
Nov 17, 2010·Respirology : Official Journal of the Asian Pacific Society of Respirology·Devipriya SubramaniyamSabina Janciauskiene
Feb 9, 2012·American Journal of Respiratory Cell and Molecular Biology·Joanna P RzepkaTanya A Miura
Apr 25, 2009·Journal of Leukocyte Biology·Johnah C GaliciaDenis F Kinane
Nov 1, 2012·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Ewa MajewskaZbigniew Baj
Jan 15, 2008·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Jerard Kneifati-HayekRita Bellevue
Nov 3, 2012·Interdisciplinary Toxicology·Tomáš PerečkoViera Jančinová
Sep 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Daocheng ZhuHongbo R Luo
May 20, 2003·Immunological Reviews·Hans-Uwe Simon
Apr 3, 2012·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Yuanning CaoE Albert Reece
Apr 19, 2015·Journal of Biomedical Science·Yi-Chinn WengWen-Hai Chou
Sep 18, 2010·Chemosphere·Hyun-Gyo Lee, Jae-Ho Yang
Oct 30, 2008·Journal of Immunotoxicology·Jared M BrownAndrij Holian
Aug 24, 2004·The Journal of Biological Chemistry·Tracie A DeVriesMary E Reyland
Feb 13, 2001·FEBS Letters·C AkgulS W Edwards
Feb 26, 2005·The Journal of Biological Chemistry·Yoon-Jin LeeYun-Sil Lee
Oct 11, 2007·American Journal of Hematology·Hongbo R Luo, Fabien Loison
Sep 18, 2009·Journal of Cellular Biochemistry·János G Filep, Driss El Kebir
Nov 10, 2009·Archiv der Pharmazie·Miao-Kun Sun, Daniel L Alkon
Jul 26, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jared M BrownAndrij Holian
Jul 26, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Erik I FinkelsteinAlbert van der Vliet
Apr 12, 2019·PLoS Pathogens·Tiffany Hensley-McBainNichole R Klatt
May 6, 2015·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·M V KuznetsovaS V Shirshev
Jun 11, 2002·American Journal of Physiology. Cell Physiology·Laurie E KilpatrickHelen M Korchak
Jul 24, 2013·The Journal of Biological Chemistry·Hua HeScheffer C G Tseng
Dec 24, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kenneth C MalcolmG Scott Worthen
Jul 31, 2019·The Journal of Clinical Investigation·Simon P JochemsDaniela M Ferreira
Dec 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ralf BaumannHans-Uwe Simon
Dec 26, 2001·The Journal of Biological Chemistry·Katalin PásztyAgnes Enyedi
Apr 20, 2000·The Journal of Biological Chemistry·S C FraschD L Bratton
Mar 3, 2007·Journal of Leukocyte Biology·Grégory SeumoisFabrice Bureau
Jul 13, 2007·American Journal of Physiology. Cell Physiology·Yingjie WangMichael G Thompson
Feb 9, 2006·Clinical Science·Stephen M BianchiMoira K B Whyte
Oct 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adnana Paunel-GörgülüMartin Scholz
Feb 24, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Justin T SchwartzLee-Ann H Allen
Jun 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·V Witko-SarsatL Halbwachs-Mecarelli
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·K SuzukiS Kitagawa
May 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mamiko MiyamotoStefan H E Kaufmann
Jul 23, 2003·Journal of Neurochemistry·Susanna KoponenJari Koistinaho
Jan 6, 2005·The Japanese Journal of Physiology·Mi Jeong KimYi-Sook Jung
Aug 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ashish NagarsekarJeffrey D Hasday
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ger van ZandbergenTamás Laskay
Jul 11, 2002·The Journal of Biological Chemistry·Chi-Ying F HuangChiun-Jye Yuan
Jul 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria Augusta ArrudaAurélio V Graça-Souza
Dec 17, 2014·The Journal of Biological Chemistry·Varun KumarWen-Hai Chou
Jul 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Keqing WangJanet M Lord
Jul 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eresso AgaTamás Laskay
Sep 9, 2006·The Journal of Pharmacology and Experimental Therapeutics·Esther P JaneIan F Pollack

Related Concepts

CASP3 protein, human
Cell Nucleus
Cell-Free System
Enzyme Activation
Neutrophil Band Cells
Protein Kinase M
Apoptosis, Intrinsic Pathway
Mitogen Activated Protein Kinase 1

Related Feeds


Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.