Spontaneous nicking in the nontoxic-nonhemagglutinin component of the Clostridium botulinum toxin complex

Biochemical and Biophysical Research Communications
Yoshimasa SaganeTohru Ohyama

Abstract

The nontoxic-nonhemagglutinin (NTNHA) component, in both isolated form and the neurotoxin (NT)/NTNHA complexed form, was prepared protease-free from toxin complexes produced by Clostridium botulinum type D strain 4947. NTNHA in both preparations was found to be spontaneously converted to the nicked NTNHA form leading to 15- and 115-kDa fragments with the excision of several amino acid residues at specific sites on SDS-PAGE during long-term incubation, while that of the NT/NTNHA/hemagglutinin complexed form remained unnicked single-chain polypeptides under the same conditions. Considering that the NTNHA preparation contained small amounts of the nicked form of NTNHA and the addition of trypsin accelerated the cleavage, it is speculated that a nicked form of NTNHA remaining after the purification and/or NTNHA itself catalyzes the cleavage of intact NTNHA.

References

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Citations

Aug 9, 2005·Applied and Environmental Microbiology·Harry B HinesErnst E Brueggemann
Apr 23, 2013·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Miles C ScotcherLarry Stanker
Feb 3, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Keita MiyataTohru Ohyama
Jun 16, 2005·Journal of Food Protection·Shashi K Sharma, Richard C Whiting
Mar 1, 2012·Biochemical and Biophysical Research Communications·Ken InuiToshihiro Watanabe

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