Spontaneous openings of NMDA receptor channels in cultured rat hippocampal neurons

The European Journal of Neuroscience
R TurecekL Vyklický

Abstract

Spontaneous and N-methyl-D-aspartate (NMDA)-evoked single-channel currents were studied in outside-out patches isolated from cultured rat hippocampal neurons. Both spontaneous and NMDA-evoked single-channel currents reversed at potentials close to 0 mV and exhibited multiple amplitude levels of similar amplitude. Both spontaneous and NMDA-evoked single-channel currents were inhibited by Mg2+ in a voltage-dependent manner and by 7-chlorokynurenic acid. The activity of spontaneous single-channel currents was reduced by the competitive NMDA receptor antagonists, but by one to three orders of magnitude less than expected assuming that the spontaneous activity is due to an ambient NMDA receptor agonist present in the extracellular solution. Our results suggest that, similar to other ligand-gated ion channels, NMDA receptor channels have a dual mode of activation--spontaneous and agonist induced.

References

Jan 1, 1990·Annual Review of Neuroscience·D W Choi, S M Rothman
Jan 1, 1990·Annual Review of Neuroscience·M Constantine-PatonE Debski
Mar 1, 1986·Biophysical Journal·M B Jackson
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J E Huettner, B P Bean
Sep 1, 1989·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·E Costa
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J A KempG N Woodruff
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M B Jackson
Aug 1, 1995·Trends in Pharmacological Sciences·T Kenakin
Jun 1, 1994·Journal of Neurophysiology·J D Clements, G L Westbrook
Jan 7, 1993·Nature·T V Bliss, G L Collingridge

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Citations

Apr 2, 1999·The European Journal of Neuroscience·J I PalecekL Vyklick
Dec 23, 2006·The Journal of Physiology·Karim Le MeurEtienne Audinat
Dec 11, 2007·Neuroscience·O CaisL Vyklicky
Dec 18, 1998·Neuron·J P Changeux, S J Edelstein
Feb 12, 2002·Nature Reviews. Neuroscience·Dean R Madden
Jan 6, 2015·The Journal of Physiology·James E Huettner

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