PMID: 6160588Oct 1, 1980Paper

Spontaneous production of human interferon

Proceedings of the National Academy of Sciences of the United States of America
L A PickeringW E Stewart

Abstract

Several established lines of human lymphoblastoid cells were evaluated for abilities to produce interferons. Some cell lines were able to produce interferon when induced with either Newcastle disease virus or Sendai virus, whereas others failed to produce detectable interferon when so induced. However, several cell lines were able to spontaneously produce interferon without induction. Spontaneously produced interferon was liberated by cells only during logarithmic growth phase, reaching levels ranging from about 10 reference units/ml of growth medium for some cell lines to 1000 reference units/ml for others. The interferons produced by induced lymphoblastoid cells and the spontaneously produced interferons were all characterized as type I human leukocyte interferon by high levels of cross-species antiviral activities on bovine cells and by neutralizations by antiserum to human leukocyte interferon but not by antiserum to human fibroblast interferon. However, analysis by electrophoresis in sodium dodecyl sulfate/polyacrylamide gels revealed that spontaneously produced interferon was less size heterogeneous than human leukocyte interferon, migrating as a single band of activity with a peak at 20,000 daltons, whereas human leukocy...Continue Reading

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Citations

Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B D WilliamsonL J Old
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R Kumar, I Atlas
Oct 1, 1986·The Journal of Cell Biology·M YaarB A Gilchrest
Feb 1, 1985·The Journal of Clinical Investigation·J L Ambrus, A S Fauci
Sep 30, 1982·Biochimica Et Biophysica Acta·P B Sehgal
Jan 1, 1982·Microbiology and Immunology·M MatsuyamaY Hinuma
Aug 1, 1982·Archives of Biochemistry and Biophysics·F H SarkarL A Pickering
Jan 1, 1982·Journal of Interferon Research·A MeagerJ Vilcek
Jan 1, 1982·Journal of Interferon Research·T Hahn, S Levin
May 1, 1992·Journal of Interferon Research·T Hahn, S Levin
Aug 1, 1992·Journal of Interferon Research·G R AdolfI Maurer-Fogy
Jan 1, 1984·Critical Reviews in Clinical Laboratory Sciences·W J Richtsmeier

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