Spontaneous recombination between homologous prophage regions causes large-scale inversions within the Escherichia coli O157:H7 chromosome

Gene
Atsushi IguchiRo Osawa

Abstract

It is known that XbaI-digested chromosomal DNAs of strains of Escherichia coli O157:H7 exhibit a wide variety in pulsed-field gel electrophoresis (PFGE) fragment patterns, which is used for epidemiological surveillance of this important pathogen. The variety in the restriction enzyme-digestion patterns suggests a wide genomic diversity, however, only a few studies have been conducted to investigate involvement of large-scale chromosomal rearrangements in development of the diversity. In this study, through rounds of subculturing E. coli O157:H7 strain EDL933, naturally occurring genome variation in the isolated derivatives was investigated. By comparing the PFGE patterns among clonally related derivatives, we found five types of large-scale inversions taking place within the chromosome. The five inversions found were across the replication axis and ranged from 250-kb to 1.4-Mb long, and all the corresponding recombination sites were associated with prophages or phage-like regions. Four inversions out of the five were resulted from recombination between pairs of lambda-like prophages disturbing the symmetry of the origin and terminus of the replication axis. These observations indicate that those prophage regions represent some ...Continue Reading

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Citations

Jan 1, 2013·Applied and Environmental Microbiology·Dongjin ParkCharles W Kaspar
Aug 29, 2007·PLoS Pathogens·Andrew E ScottIan F Connerton
Jan 24, 2014·Japanese Journal of Infectious Diseases·Kayo OsawaToshiro Shirakawa
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Feb 10, 2016·Frontiers in Microbiology·Jens A HammerlStefan Hertwig
Jun 13, 2015·Molecular Plant Pathology·Claudia BartoliJay Ram Lamichhane
Jan 22, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Durga Prasad DasS B Barbuddhe
Oct 13, 2016·Cellular and Molecular Life Sciences : CMLS·Susan SchlegelJan-Willem de Gier
Jun 20, 2017·PLoS Genetics·Matteo CossuJacques Oberto
May 29, 2007·Microbiology·Michael L KotewiczThomas A Cebula
Feb 28, 2019·Frontiers in Genetics·Bhaskar Chandra Mohan Ramisetty, Pavithra Anantharaman Sudhakari
Mar 13, 2021·Current Biology : CB·Allison M Murawski, Mark P Brynildsen
Nov 10, 2021·Microbial Genomics·Stephen F FitzgeraldDavid L Gally

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