Spontaneous Reconstitution of Functional Transmembrane Proteins During Bioorthogonal Phospholipid Membrane Synthesis

Angewandte Chemie
Christian M ColeNeal K Devaraj

Abstract

Transmembrane proteins are critical for signaling, transport, and metabolism, yet their reconstitution in synthetic membranes is often challenging. Non-enzymatic and chemoselective methods to generate phospholipid membranes in situ would be powerful tools for the incorporation of membrane proteins. Herein, the spontaneous reconstitution of functional integral membrane proteins during the de novo synthesis of biomimetic phospholipid bilayers is described. The approach takes advantage of bioorthogonal coupling reactions to generate proteoliposomes from micelle-solubilized proteins. This method was successfully used to reconstitute three different transmembrane proteins into synthetic membranes. This is the first example of the use of non-enzymatic chemical synthesis of phospholipids to prepare proteoliposomes.

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Citations

Jul 22, 2016·Proceedings of the National Academy of Sciences of the United States of America·Roberto J BreaNeal K Devaraj
Mar 7, 2017·Journal of the American Chemical Society·Roberto J BreaNeal K Devaraj
May 4, 2017·The Journal of Organic Chemistry·Neal K Devaraj
Apr 6, 2018·Chemical Science·Ahanjit BhattacharyaNeal K Devaraj
Apr 2, 2019·Angewandte Chemie·Jorge RoyesChristophe Tribet
Jan 19, 2019·Nature Communications·Ahanjit BhattacharyaNeal K Devaraj
May 26, 2017·Nature Communications·Helen M BurkeEoin M Scanlan
Nov 11, 2019·Emerging Topics in Life Sciences·Marten Exterkate, Arnold J M Driessen
May 13, 2021·Journal of the American Chemical Society·Satyam KhanalNeal K Devaraj
May 21, 2021·Journal of the American Chemical Society·Jacob A Vance, Neal K Devaraj
Nov 30, 2018·Journal of the American Chemical Society·Roberto J BreaNeal K Devaraj
Apr 6, 2019·ACS Omega·Marten Exterkate, Arnold J M Driessen

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