Spontaneous synaptic activity is required for the formation of functional GABAergic synapses in the developing rat hippocampus

The Journal of Physiology
Isabelle Colin-Le BrunJean-Luc Gaïarsa

Abstract

Here we examine the role of the spontaneous synaptic activity generated by the developing rat hippocampus in the formation of functional gamma-aminobutyric acid (GABA) synapses. Intact hippocampal formations (IHFs) were dissected at birth and incubated for 1 day in control or tetrodotoxin (TTX)-supplemented medium at 25 degrees C. After the incubation, miniature GABA(A)-mediated postsynaptic currents (mGABA(A)-PSCs) were recorded in whole-cell voltage-clamped CA3 pyramidal neurones from IHF-derived slices. After 1 day in vitro in control medium, the frequency of mGABA(A)-PSCs was similar to that recorded in acute slices obtained 1 day after birth, but significantly higher than the frequency recorded from acute slices just after birth. These results suggest that the factors required in vivo for the formation of functional GABAergic synapses are preserved in the IHFs in vitro. The frequency increase was prevented when IHFs were incubated for 1 day with TTX. TTX treatment affected neither the morphology of CA3 pyramidal neurones nor cell viability. The TTX effects were reproduced when IHFs were incubated in the presence of glutamatergic or GABAergic ionotropic receptor antagonists or in high divalent cationic medium. The present r...Continue Reading

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Citations

Jun 13, 2008·Journal de la Société de biologie·Nicolas Le RouxPhilippe Fossier
Jul 17, 2008·Epilepsia·Chuan-Sheng Zhao, Linda Overstreet-Wadiche
May 14, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Stefano Zucca, C Fernando Valenzuela
Feb 25, 2014·Frontiers in Neuroendocrinology·David J Lyons, Christian Broberger
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Sep 20, 2008·The Journal of Physiology·Victoria F SafiulinaEnrico Cherubini
Jul 2, 2011·Pharmacology & Therapeutics·Jean-Luc GaiarsaChristophe Porcher
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Apr 3, 2008·Developmental Neurobiology·Timothy A Dunn, Marla B Feller
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Jun 20, 2019·Frontiers in Cellular Neuroscience·Camille DumonJean-Luc Gaiarsa
Feb 8, 2021·Neuroscience and Biobehavioral Reviews·Carlijn Peerboom, Corette J Wierenga

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