PMID: 173708Feb 1, 1976

Spore membrane(s) as the site of damage within heated Clostridium perfringens spores

Journal of Bacteriology
R S Flowers, D M Adams

Abstract

Clostridium perfringens spores were injured by ultrahigh-temperature treatment at 105 C for 5 min. Injury was manifested as an increased sensitivity to polymyxin and neomycin. Since many of the survivors could not germinate normally the ultrahigh-temperature-treated spores were sensitized to and germinated by lysozyme. Polymyxin reportedly acts upon the cell membrane. Neomycin may inhibit protein synthesis and has surface-active properties. Injured spores were increasingly sensitive to known surface-active agents, sodium lauryl sulfate, sodium deoxycholate, and Roccal, a quaternary ammonium compound. Injured spores sensitive to polymyxin and neomycin also were osmotically fragile and died during outgrowth in a liquid medium unless the medium was supplemented with 20% sucrose, 10% dextran, or 10% polyvinylpyrrolidone. The results suggested that a spore structure destined to become cell membrane or cell wall was the site of injury. Repair of injury during outgrowth in the presence of protein, deoxyribonucleic acid, ribonucleic acid and cell wall synthesis inhibitors was consistent with this hypothesis.

Citations

Apr 1, 1983·The Journal of Applied Bacteriology·Y Ando, T Tsuzuki
Jan 15, 1993·FEMS Microbiology Letters·N D Williams, A D Russell
Jul 4, 2003·Science Progress·A D Russell
Jan 1, 1994·Society for Applied Bacteriology Symposium Series·R E MarquisS Y Shin
Nov 8, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Irene O L McHugh, Ayly Ling Tucker

Related Concepts

Osvan
Plasma Membrane
Clostridium perfringens
Deoxycholic Acid, Sodium Salt, 12beta-Isomer
Antibiotic Resistance, Microbial
Leftose
Neomycin Palmitate
Osmotic Fragility Test
Polymyxin M
Irium

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