PMID: 9440380Jan 24, 1998Paper

Spot ferritin assay for serum samples dried on filter paper

The American Journal of Clinical Nutrition
N AhluwaliaL H Allen

Abstract

A spot method was developed for analyzing ferritin from 20-microL serum samples (n = 71) that were spotted and dried on filter paper and stored frozen (2 d). Spot samples were thawed, incubated in a buffer containing cellulase, and centrifuged and the supernate assayed for ferritin by a commercial radioimmunoassay. The geometric means (+/- 1 SD) for ferritin analyzed with the spot and traditional methods were 49.4 (range: 14.9-164.0) and 47.5 (range: 14.4-156.0) micrograms/L, respectively. The two methods correlated strongly (r = 0.98, P = 0.0001). Storage of spot samples (n = 31) under various conditions (at room temperature, refrigerated, or frozen for 2 wk, or at room temperature for 4 wk) in airtight bags before analysis yielded ferritin values that were not significantly different from those obtained by the traditional method. Ferritin values from spotted samples stored at room temperature for 4 wk before being analyzed were only 2.2 micrograms/L higher than those from samples analyzed by the traditional method. With iron depletion defined as a serum ferritin concentration < 15 micrograms/L, this method corresponded absolutely with the traditional method in classifying individuals as iron sufficient or deficient. Thus, the...Continue Reading

Citations

Jun 6, 2009·Paediatric and Perinatal Epidemiology·Richard Jones, Jean Golding
May 9, 2000·The American Journal of Clinical Nutrition·J L Beard
Oct 26, 2002·The American Journal of Clinical Nutrition·Joel Monárrez-Espino, Ted Greiner
Oct 17, 2013·American Journal of Human Biology : the Official Journal of the Human Biology Council·Thomas W McDade
Jan 30, 2002·The American Journal of Clinical Nutrition·Namanjeet AhluwaliaRoshni Molls
Jul 17, 2012·Advances in Nutrition·Caitlin R CrowleyKlaus Schümann
Feb 15, 2020·European Journal of Haematology·Karsten KoehlerJiri Adamec
Oct 21, 2003·Food and Nutrition Bulletin·Rainer Gross, Noel W Solomons

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