Abstract
In order to map antigenic domains on the P-protein of morbillivirus, a series of overlapping peptides, representing the P-protein sequences of phocid distemper virus strain 2558/Han88 and canine distemper virus strain Onderstepoort, were synthesized on a paper support by the spot-technique. The reactivity of six monoclonal antibodies with the peptides was tested in an enzyme immunoassay and compared to their reactivity in Western blots and in an ELISA using detergent extracts from virus-infected cells. Three linear determinants could be localized on the P-protein. Two antibody-binding sites were delineated within the C-terminal (between amino acids 307-322 and 382-400, respectively), and a third one was located on the N-terminal part (amino acids 13-31) of the protein. Fine mapping of this binding site revealed that this was a part of an antigenic domain. In Western blots, the monoclonal antibodies reacting with this domain also reacted with a second protein which was possibly the V-protein.
References
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 1, 1992·The Journal of General Virology·M D Curran, B K Rima
Apr 1, 1992·The Journal of General Virology·M Blixenkrone-MöllerJ Kövamees
Sep 1, 1990·The Journal of General Virology·C OrvellP Have
Jan 1, 1991·Archives of Virology·T C HarderB Liess
Sep 24, 1987·Journal of Immunological Methods·H M GeysenP G Schoofs
Mar 1, 1985·The Journal of General Virology·C OrvellE Norrby
Nov 1, 1985·Virus Research·T BarrettS E Russell
Aug 15, 1970·Nature·U K Laemmli
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H M GeysenS J Barteling
Jan 1, 1993·Journal of Virological Methods·T C HarderB Liess
Citations
Apr 6, 2004·International Journal for Parasitology·E PrecigoutA Gorenflot
Sep 19, 2002·Protein Science : a Publication of the Protein Society·Xavier EspanelRob Hooft van Huijsduijnen
Apr 22, 1999·Protein Science : a Publication of the Protein Society·M ValleJ P Albar
Jun 20, 2001·Journal of Virology·V von MesslingR Cattaneo
Jul 29, 2011·Cellular Microbiology·Can ÜnalMichael Steinert
Dec 19, 2009·Biochimica Et Biophysica Acta·Christian K NicklWolfgang R Dostmann
Aug 8, 2012·Virology·Denise MeyerMartin A Hofmann
Oct 19, 2006·Virology·Jean-Paul RivalsRiccardo Wittek
Jul 3, 2013·Journal of Virological Methods·Zhiguang XiangQiang Wei
May 17, 2005·Virology·Philippe PlattetRiccardo Wittek
Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Kai HilperRobert E W Hancock
Jan 1, 2015·Microarrays·Sarah Picaud, Panagis Filippakopoulos
Oct 10, 1998·Journal of Virology·H LiebermannW Seidel
Oct 9, 2004·BioTechniques·Luo SunMing-Qun Xu