SPP1, analyzed by bioinformatics methods, promotes the metastasis in colorectal cancer by activating EMT pathway

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Chunjie XuQing Xu

Abstract

Tumor metastasis is still a great challenge for the prognosis of colorectal cancer (CRC). Although secreted phosphoprotein 1 (SPP1) over-expression is confirmed to associate with invasion, metastasis of CRC, the underlying mechanism by which modulates the CRC metastasis is still not fully explained. GDS4382 was obtained from GEO database and differentially expressed genes (DEGs) were analyzed by bioinformatics methods 55 paired samples of CRC and adjacent non-cancerous tissues were collected to detect the expression of SPP1 by q-PCR and western blot. Functional analysis of siRNA-SPP1, including proliferation, apoptosis, colony formation, cell cycle, migration, was investigated in CRC cell lines and tumor xenografts were conducted in nude mice. Protein expression of E-cadherin and vimentin was detected by western blot. 1887 DEGs were analyzed and selected from GDS4382, of which, SPP1 and epithelial-mesenchymal-transition (EMT) showed a close association by bioinformatics analysis. The mRNA and protein expression of SPP1 were significantly higher in CRC tissues than that in adjacent non-cancerous tissues (P<0.05). Overexpression of SPP1 closely associated with tumor invasion, metastasis and low survival in CRC. Moreover, siRNA-SP...Continue Reading

Citations

Apr 24, 2018·Giornale Italiano Di Dermatologia E Venereologia : Organo Ufficiale, Società Italiana Di Dermatologia E Sifilografia·Max RybarskiThomas Dirschka
Apr 9, 2020·Biomolecules·Elena Sánchez-LuisJavier De Las Rivas
Aug 23, 2020·Cancers·Alexandra Elisabeth WagnerRoland Kappler
Dec 10, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Qiaowei FanHulun Li
Sep 4, 2021·Therapeutics and Clinical Risk Management·Ya-Fei QinHao Wang
Dec 29, 2021·International Journal of Environmental Health Research·Liu YangQiao Zhang

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