Spray-dried powders enhance vaginal siRNA delivery by potentially modulating the mucus molecular sieve structure

International Journal of Nanomedicine
Na WuJuan Li

Abstract

Vaginal small interfering RNA (siRNA) delivery provides a promising strategy for the prevention and treatment of vaginal diseases. However, the densely cross-linked mucus layer on the vaginal wall severely restricts nanoparticle-mediated siRNA delivery to the vaginal epithelium. In order to overcome this barrier and enhance vaginal mucus penetration, we prepared spray-dried powders containing siRNA-loaded nanoparticles. Powders with Pluronic F127 (F127), hydroxypropyl methyl cellulose (HPMC), and mannitol as carriers were obtained using an ultrasound-assisted spray-drying technique. Highly dispersed dry powders with diameters of 5-15 μm were produced. These powders showed effective siRNA protection and sustained release. The mucus-penetrating properties of the powders differed depending on their compositions. They exhibited different potential of opening mesh size of molecular sieve in simulated vaginal mucus system. A powder formulation with 0.6% F127 and 0.1% HPMC produced the maximum increase in the pore size of the model gel used to simulate vaginal mucus by rapidly extracting water from the gel and interacting with the gel; the resulting modulation of the molecular sieve effect achieved a 17.8-fold improvement of siRNA del...Continue Reading

Citations

Feb 2, 2016·Advanced Drug Delivery Reviews·José das NevesBruno Sarmento
May 27, 2020·ACS Applied Materials & Interfaces·Avinash Chandra KushwahaSubhasree Roy Choudhury

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Methods Mentioned

BETA
dynamic light scattering
electrophoresis
rheology
confocal microscopy

Software Mentioned

SPSS
ImageJ

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