Spreading of B16 F1 cells on laminin and its proteolytic fragments P1 and E8: involvement of laminin carbohydrate chains

Experimental Cell Research
M BouzonP M Martin

Abstract

The properties of EHS laminin and its proteolytic fragments E8 and P1 to promote spreading of B16 F1 murine melanoma cells were studied in short-term adhesion assays. The cells exhibited similar attachment rates but distinct spread morphologies on laminin, P1, and E8 fragments. The extent of spreading and the shape of the cells were quantitatively defined by two geometrical parameters: the surface and the form factor. These parameters were computed with an automatic image analyzer. Wheat germ agglutinin (WGA), applied to laminin-coated substrates, totally blocked cell spreading, but did not modify attachment percentages. Under similar conditions, WGA partially inhibited cell spreading on the E8 fragment and had no effect on the P1 fragment. In Western blot analysis, P1 fragment, contrary to laminin and E8, did not bind WGA. Laminin galactosylation and cell treatment with alpha-lactalbumin, which should prevent cell galactosyltransferase (GalTase) from binding to N-acetylglucosamine (GlcNAc) residues of the substrate, had no effect on the spreading ability of B16 F1 cells. The role of laminin N-linked carbohydrate chains in the induction of B16 F1 cell spreading was studied further after endoglycosidase F (Endo F) treatment of t...Continue Reading

References

Jan 1, 1978·Advances in Carbohydrate Chemistry and Biochemistry·I J Goldstein, C E Hayes
Jan 1, 1990·The Journal of Cell Biology·B ClémentY Yamada
Aug 1, 1989·The Journal of Cell Biology·S L GoodmanK von der Mark
Jul 31, 1989·Biochemical and Biophysical Research Communications·G C SephelH K Kleinman
Nov 1, 1986·The Journal of Cell Biology·G E JonesM L Tanzer
Oct 14, 1988·Biochemical and Biophysical Research Communications·J W DeanM L Tanzer
Jan 1, 1987·Annual Review of Cell Biology·D R McClay, C A Ettensohn
Jan 1, 1985·Journal of Cellular Biochemistry·H K KleinmanM DuBois-Dalcq
Nov 1, 1983·Archives of Biochemistry and Biophysics·C N RaoL A Liotta
Mar 1, 1982·European Journal of Biochemistry·U OttR Timpl
Sep 1, 1981·European Journal of Biochemistry·A R CooperB L Hogan

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Citations

Jun 1, 1991·Journal of Cellular Biochemistry·S ChandrasekaranM L Tanzer
Feb 25, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·B B GowenE P Mayer
Dec 1, 1991·The Journal of Cell Biology·D N CooperS H Barondes
Oct 1, 1994·In Vitro Cellular & Developmental Biology. Animal·J TurnayM A Lizarbe
Jan 1, 1993·Kidney International·M L TanzerM S Giniger
Nov 10, 1992·Biochemistry·J Engel

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