Sprouty2 mediated tuning of signalling is essential for somite myogenesis

BMC Medical Genomics
Muhammad Abu-ElmagdAndrea Münsterberg

Abstract

Negative regulators of signal transduction cascades play critical roles in controlling different aspects of normal embryonic development. Sprouty2 (Spry2) negatively regulates receptor tyrosine kinases (RTK) and FGF signalling and is important in differentiation, cell migration and proliferation. In vertebrate embryos, Spry2 is expressed in paraxial mesoderm and in forming somites. Expression is maintained in the myotome until late stages of somite differentiation. However, its role and mode of action during somite myogenesis is still unclear. Here, we analysed chick Spry2 expression and showed that it overlaps with that of myogenic regulatory factors MyoD and Mgn. Targeted mis-expression of Spry2 led to inhibition of myogenesis, whilst its C-terminal domain led to an increased number of myogenic cells by stimulating cell proliferation. Spry2 is expressed in somite myotomes and its expression overlaps with myogenic regulatory factors. Overexpression and dominant-negative interference showed that Spry2 plays a crucial role in regulating chick myogenesis by fine tuning of FGF signaling through a negative feedback loop. We also propose that mir-23, mir-27 and mir-128 could be part of the negative feedback loop mechanism. Our analy...Continue Reading

References

Dec 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·V Hamburger, H L Hamilton
May 1, 1995·Anatomy and Embryology·B Christ, C P Ordahl
Mar 30, 2001·The Journal of Biological Chemistry·Y YigzawT B Patel
Jul 13, 2001·Current Opinion in Genetics & Development·M Buckingham
Oct 29, 2002·Nature Cell Biology·Hiroshi HanafusaEisuke Nishida
May 2, 2003·Nature Cell Biology·Gerhard Christofori
Jun 20, 2003·Current Biology : CB·Maxwell C EblaghieCheryll Tickle
Sep 12, 2003·Developmental Cell·Lúcia E AlvaresSusanne Dietrich
Mar 3, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Maike SchmidtAndrea Münsterberg
Jun 3, 2004·Nature Reviews. Molecular Cell Biology·Hong Joo Kim, Dafna Bar-Sagi
Sep 8, 2004·Biochemical and Biophysical Research Communications·Helen M PoppletonTarun B Patel
Sep 24, 2004·Developmental Biology·Louise ChengSusanne Dietrich
Jan 18, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Chunxiang ZhangTarun B Patel
Feb 5, 2005·Developmental Cell·M Albert BassonJonathan D Licht
Jul 8, 2005·Molecular Biology of the Cell·Cristina de AlvaroMargarita Lorenzo
Dec 13, 2005·Trends in Cell Biology·Jacqueline M MasonJonathan D Licht
Jun 29, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Dylan SweetmanTamas Dalmay
Jan 26, 2008·Angiogenesis·Miguel A Cabrita, Gerhard Christofori
May 30, 2008·Molecular Biology of the Cell·Danish SayedMaha Abdellatif
Apr 23, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sophie Eloy-TrinquetDelphine Duprez
Aug 18, 2009·Molecular and Cellular Neurosciences·Barbara HausottLars Klimaschewski
Oct 24, 2009·Developmental Biology·Muhammad Abu-ElmagdAndrea Münsterberg
Mar 20, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Andrea FaedoJohn L R Rubenstein
Dec 25, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Gi Fay Mok, Dylan Sweetman
May 17, 2011·Nature·Anne C RiosChristophe Marcelle

❮ Previous
Next ❯

Citations

Oct 21, 2020·Developmental Biology·Camille ViautAndrea Münsterberg

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

SPSS
Axiovision
Targetscan

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.