Sprouty2 suppresses progression and correlates to favourable prognosis of intrahepatic cholangiocarcinoma via antagonizing FGFR2 signalling

Journal of Cellular and Molecular Medicine
Yun-Fei XuJin-Ming Yu

Abstract

Fibroblast growth factor receptor 2 (FGFR2) was demonstrated to correlate to the progression and prognosis of intrahepatic cholangiocarcinoma (ICC) by numerous evidences. However, as a well-recognized suppressor of FGFR2 signalling, the clinical significance of Sprouty (SPRY) family of ICC has not been investigated. In our study, the expressions of SPRY1-4 in 20 pairs of fresh tumour tissues were detected with qPCR, and in 108 cases of paraffin-embedded tissues with immunohistochemistry. The prognostic value of SPRY family in ICC was estimated with univariate analysis and multivariate analysis. As a result, SPRY2 was identified as an independent prognostic biomarker predicting favourable prognosis of ICC. High SPRY2 expression was correlated with good differentiation of ICC. With silencing SPRY2 expression, we demonstrated that SPRY2 could suppress FGFR2-induced ERK phosphorylation, migration, invasion and epithelial-mesenchymal transition (EMT) under FGF1 stimulation. By overexpressing SPRY2-wide type or SPRY2-Y55F, the tyrosine-55 of SPRY2 was demonstrated to be essential in suppressing ERK phosphorylation, tumour invasion and EMT of ICC cells. In conclusion, SPRY2 was correlated with favourable prognosis of ICC via suppressi...Continue Reading

References

Oct 29, 2002·Nature Cell Biology·Hiroshi HanafusaEisuke Nishida
Apr 30, 2003·Nature Cell Biology·Atsuo SasakiAkihiko Yoshimura
Sep 21, 2011·Journal of Surgical Oncology·Yongyut SirivatanauksornChalita Tongkham
Mar 6, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·A Nigel BrooksPaul D Smith
Feb 26, 2013·The Journal of Clinical Investigation·Rachana PatelHing Y Leung
Apr 6, 2013·Cancer Discovery·Yi-Mi WuArul M Chinnaiyan
Oct 15, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yasuhito AraiTatsuhiro Shibata
Jan 29, 2014·Oncogene·D G R YimD M Virshup
Feb 26, 2014·Biochemical and Biophysical Research Communications·Yun-Fei XuYu-Xin Chen
Mar 4, 2014·Lancet·Nataliya Razumilava, Gregory J Gores
Apr 20, 2014·Cancer Metastasis Reviews·Samar Masoumi-MoghaddamDavid Lawson Morris
Mar 26, 2015·World Journal of Gastroenterology : WJG·Yun-Fei XuJie Yang
Jan 31, 2016·The Journal of Biological Chemistry·Sumera RizviGregory J Gores

❮ Previous
Next ❯

Citations

Sep 5, 2019·Irish Journal of Medical Science·Xue DingZijia Li
Apr 3, 2020·International Journal of Oncology·Yu-Xue GaoDe-Xi Chen
Mar 16, 2019·Cancer Science·Tetsuro Kawazoe, Koji Taniguchi
Feb 18, 2020·Irish Journal of Medical Science·Mei-Yuan ChenMing Ma
Jul 4, 2020·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·J PengR Zeng
May 10, 2020·Cell Death & Disease·Kang-Shuai LiHui-Chuan Sun
Feb 19, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Tianli ChenZongli Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical
PCR
transfection
xenograft

Software Mentioned

SPSS

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.