SPR/SERS dual-mode plasmonic biosensor via catalytic hairpin assembly-induced AuNP network.

Biosensors & Bioelectronics
Chunyuan SongLianhui Wang

Abstract

Highly sensitive and reliable detection of disease-related nucleic acids is still a big challenge in liquid biopsy because of their homologous sequences and low abundance. Herein, a novel surface plasmon resonance/surface-enhanced Raman scattering (SPR/SERS) dual-mode plasmonic biosensor based on catalytic hairpin assembly (CHA)-induced Au nanoparticle (AuNP) network was proposed for highly sensitive and reliable detection of cancer-related miRNA-652. The biosensor includes capture DNA-functionalized AuNPs (Probe 1), H1 and 4-mercaptobenzoic acid (4-MBA) co-modified AuNPs (Probe 2), and 6-carboxyl-Xrhodamine (ROX)-labeled H2 (fuel strands). The Probe 1-Probe 2 networks were formed via the target-triggered CHA reactions, which resulted in the color change of dark-field microscopy (DFM) images and enhanced SERS effect. The SPR sensing was achieved by extracting the integral optical density of dark-field color in DFM images, and the SERS sensing was realized by the ratiometric SERS signals of ROX and internal standards 4-MBA molecules. After characterizing the feasibility and optimality of the sensing strategy, the good performance of biosensors on sensitivity, specificity and uniformity was approved. The practicability of biosens...Continue Reading

References

May 24, 2003·Trends in Molecular Medicine·Simone MocellinFrancesco M Marincola
Mar 4, 2008·Optics Letters·Jing GuoJ T Hastings
Sep 1, 2011·Nature Reviews. Genetics·Zuben E Sauna, Chava Kimchi-Sarfaty
May 23, 2012·Applied Optics·G DyankovM Bousmina
Oct 20, 2012·Nature Reviews. Drug Discovery·Eva van Rooij, Eric N Olson
Jan 1, 2013·Chemical Reviews·Yunqing WangLingxin Chen
Nov 10, 2015·Chemical Reviews·Yongxi ZhaoChunhai Fan
Dec 15, 2015·Analytical Chemistry·Richard M Graybill, Ryan C Bailey
Mar 6, 2018·Journal of the American Chemical Society·Ying ZhangLianhui Wang
May 20, 2018·Proceedings of the National Academy of Sciences of the United States of America·Klaas E A MaxThomas Tuschl
Feb 11, 2020·Chemical Communications : Chem Comm·Xuan-Xuan ZhangWei Ma
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yi HeYaqin Chai
Sep 16, 2020·Proceedings of the National Academy of Sciences of the United States of America·Hirosha GeekiyanageVictor Ambros

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