Sputum Mycobacterium tuberculosis mRNA as a marker of bacteriologic clearance in response to antituberculosis therapy.

Journal of Clinical Microbiology
L LiKathleen D Eisenach

Abstract

mRNA is a marker of cell viability. Quantifying Mycobacterium tuberculosis mRNA in sputum is a promising tool for monitoring response to antituberculosis therapy and evaluating the efficacy of individual drugs. mRNA levels were measured in sputum specimens from patients with tuberculosis (TB) receiving monotherapy in an early bactericidal activity study of fluoroquinolones and in those receiving a standard rifampin-based regimen in an interleukin-2 (IL-2) trial. In the early bactericidal activity study, sputum for quantitative culture and mRNA analysis was collected for 2 days before and daily during 7 days of study drug administration. In the IL-2 trial, sputum was collected for quantitative culture, Bactec 460 liquid culture, and mRNA analysis throughout the intensive treatment phase. RNA was isolated from digested sputum and tested in quantitative reverse transcription-PCR assays for several gene targets. mRNA for the glyoxylate cycle enzyme isocitrate lyase declined at similar rates in patients receiving isoniazid, gatifloxicin, levofloxacin, and moxifloxacin monotherapy. Isocitrate lyase mRNA correlated highly with CFU in sputum prior to therapy and during 7 days of monotherapy in all treatment arms. Isocitrate lyase mRNA ...Continue Reading

References

Apr 1, 1993·The American Review of Respiratory Disease·D A Mitchison
Nov 1, 1996·Journal of Clinical Microbiology·T J HellyerK D Eisenach
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y YuanC E Barry
Jan 16, 1999·Journal of Clinical Microbiology·T J HellyerK D Eisenach
Jul 3, 1999·American Journal of Respiratory and Critical Care Medicine·L E DesjardinK D Eisenach
Jun 16, 2001·Antimicrobial Agents and Chemotherapy·R DietzeJ J Ellner
Jan 29, 2003·The Journal of Infectious Diseases·Robert S WallisJerrold J Ellner
Apr 19, 2003·American Journal of Respiratory and Critical Care Medicine·John L JohnsonUNKNOWN Uganda-Case Western Reserve University Research Collaboration
Nov 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Juliano TimmJohn D McKinney
Oct 16, 2004·Expert Review of Anti-infective Therapy·P R DonaldP B Fourie

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Citations

Jan 17, 2014·The Lancet. Respiratory Medicine·Sven O FriedrichUNKNOWN Pan African Consortium for the Evaluation of Anti-tuberculosis Antibiotics (PanACEA)
Mar 27, 2012·The Journal of Infectious Diseases·Patrick P J PhillipsMichael Hoelscher
Mar 27, 2012·The Journal of Infectious Diseases·Peter S KimRichard Hafner
Nov 2, 2013·PLoS Neglected Tropical Diseases·Grace L DavisLinda B Adams
May 9, 2014·PLoS Pathogens·Catherine VilchèzeLaurent Kremer
Jun 19, 2014·American Journal of Respiratory and Critical Care Medicine·Jan HeyckendorfChristoph Lange
Sep 8, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A H DiaconP R Donald
Dec 15, 2015·The Journal of Antimicrobial Chemotherapy·Romelie RieuRobert N Davidson
Apr 1, 2016·Expert Review of Respiratory Medicine·Neesha RockwoodRobert J Wilkinson
Mar 13, 2015·The Journal of Infectious Diseases·Nicholas D WalterJohn Lucian Davis
Dec 30, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Stephen D Lawn, Mark P Nicol
Dec 24, 2014·Tuberculosis·Vladyslav NikolayevskyyFrancis Drobniewski
Feb 20, 2018·Respirology : Official Journal of the Asian Pacific Society of Respirology·Delia GolettiTom H M Ottenhoff
May 30, 2014·Journal of Clinical Microbiology·Isobella HoneyborneUNKNOWN Pan African Consortium for the Evaluation of Anti-tuberculosis Antibiotics
Oct 27, 2018·The European Respiratory Journal·Delia GolettiFrank Cobelens
Aug 9, 2012·Archives of Gynecology and Obstetrics·Otto May FeuerschuetteRosemeri Maurici da Silva
Jan 8, 2014·Expert Review of Anti-infective Therapy·Andreas H Diacon, Peter R Donald
Feb 8, 2019·Journal of Clinical Microbiology·Wilber SabiitiStephen H Gillespie
Aug 30, 2020·British Journal of Pharmacology·Rob C van WijkElke H J Krekels

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