PMID: 41173Oct 15, 1979

Squalene synthetase

Molecular and Cellular Biochemistry
G Popják, W S Agnew

Abstract

In the first part of the review the background to the discovery of the asymmetric synthesis of squalene from two molecules of farnesyl pyrophosphate and NADPH is described, then the stereochemistry of the overall reaction is summarized. The complexity of the biosynthesis of squalene by microsomal squalene synthetase demanded the existence of some intermediate(s) between farnesyl pyrophosphate and squalene. This demand was satisfied by the discovery of presqualene pyrophosphate, an optically active C30 substituted cyclopropylcarbinyl pyrophosphate, the absolute configuration of which at all three asymmetric centers of the cyclopropane ring was deduced to be R. Possible mechanisms for the biosynthesis of presqualene pyrophosphate and its reductive transformation into squalene are presented. In the second part of the review the nature of the enzyme is discussed. The question whether presqualene pyrophosphate is an obligate intermediate in the biosynthesis of squalene is examined, with the firm conclusion that it is. It is as yet uncertain whether the two half reactions of squalene synthesis, i.e. (i) 2 x farnesyl pyrophosphate leads to presqualene pyrophosphate; (ii) presqualene pyrophosphate + NADPH (NADH) leads to squalene, are ...Continue Reading

References

Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S M JenningsG W Robinson
Jul 13, 2002·Plant Physiology·Timothy P DevarenneJoe Chappell
Jan 2, 2013·The International Journal of Biochemistry & Cell Biology·Jian YangShen-Jiang Hu
Sep 25, 2012·Analytical Biochemistry·Huaxiang TongRaymond J Hohl
May 7, 2008·Analytical Biochemistry·Huaxiang TongRaymond J Hohl
Feb 24, 2004·Cardiovascular Drug Reviews·Kristy L West, Maria Luz Fernandez
Jul 15, 2000·The Journal of Biological Chemistry·J PanditH J Harwood

Related Concepts

Terpene Phosphates
Oxidase
Yeasts
Farnesol
Microsomes, Liver
Substrate Specificity
Phospholipids
NADP
Farnesyl-diphosphate farnesyltransferase
FDFT1

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