Squaramides and Ureas: A Flexible Approach to Polymerase-Compatible Nucleic Acid Assembly.

Angewandte Chemie
Arun ShivalingamTom Brown

Abstract

Joining oligonucleotides together (ligation) is a powerful means of retrieving information from the nanoscale. To recover this information, the linkages created must be compatible with polymerases. However, enzymatic ligation is restrictive and current chemical ligation methods lack flexibility. Herein, a versatile ligation platform based on the formation of urea and squaramide artificial backbones from minimally modified 3'- and 5'-amino oligonucleotides is described. One-pot ligation gives a urea linkage with excellent read-through speed, or a squaramide linkage that is read-through under selective conditions. The squaramide linkage can be broken and reformed on demand, while stable pre-activated precursor oligonucleotides expand the scope of the ligation reaction to reagent-free, mild conditions. The utility of our system is demonstrated by replacing the enzymatically biased RNA-to-DNA reverse transcription step of RT-qPCR with a rapid nucleic-acid-template-dependent DNA chemical ligation system, that allows direct RNA detection.

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Apr 18, 2018·Chemical Communications : Chem Comm·Ilenia ManuguerraAntonio Manetto

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Citations

Feb 27, 2021·Molecular Cancer·Lei MiaoLeaf Huang
Sep 22, 2021·Journal of the American Chemical Society·Sven EppleTom Brown
Sep 15, 2021·Chembiochem : a European Journal of Chemical Biology·Kazuki YamaokaHiroshi Abe

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Methods Mentioned

BETA
nucleic acid ligation
PCR
in vitro transcription

Software Mentioned

ImageJ
BLAST
Vent

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