SR-FTIR as a tool for quantitative mapping of the content and distribution of extracellular matrix in decellularized book-shape bioscaffolds

BMC Musculoskeletal Disorders
Yongchun ZhouHongbin Lu

Abstract

To evaluate synchrotron radiation-based Fourier transform infrared microspectroscopy (SR-FTIR) as a tool for quantitative mapping of the content and distribution of the extracellular matrix in decellularized fibrocartilage bioscaffolds, and to provide a new platform for quantitatively characterizing bioscaffolds for tissue engineering. Fibrocartilage was harvested and cut into book-shape bioscaffolds (N = 54), which were then decellularized. The structures and distribution of collagen fibrous and intrinsic ultrastructure in decellularized fibrocartilage bioscaffolds were evaluated by histological staining and scanning electron microscopy (SEM), respectively. The content of collagen and proteoglycan in the cellularized or decellularized bioscaffolds were also measured by SR-FTIR and biochemical assay. Book-shape fibrocartilage decellularized bioscaffolds were successfully obtained. Histological examination revealed that the structure of extracellular matrix endured during decellularization. Histology and DNA quantification analysis confirmed substantial removal of cells during decellularization. SEM demonstrated that intrinsic ultrastructure of the fibrocartilage bioscaffold was also well preserved. SR-FTIR quantitative analysis...Continue Reading

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Citations

Jan 14, 2020·Biomaterials Science·Juhi ChakrabortySourabh Ghosh
Dec 20, 2019·Stem Cells and Development·Tahera AnsariPaul D Sibbons
Mar 11, 2020·Polymers·Andrea Catalina Villamil BallesterosYuli Alexandra Neira Bejarano
Mar 27, 2021·Cell and Tissue Banking·Ravi Prakash GoyalNaveen Kumar

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Methods Mentioned

BETA
infrared microspectroscopy
scanning electron microscopy
infrared spectroscopy
light microscopy
imaging technique
X-ray

Software Mentioned

SPSS
Omnic
SPSS13

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