Sr2+ supports depolarization-induced suppression of inhibition and provides new evidence for a presynaptic expression mechanism in rat hippocampal slices

The Journal of Physiology
W Morishita, B E Alger

Abstract

1. We studied the transient suppression of evoked GABAA ergic inhibitory postsynaptic currents (eIPSCs) that follows brief membrane depolarization in rat CA1 hippocampal pyramidal cells, a process called depolarization-induced suppression of inhibition (DSI). We used whole-cell patch electrodes filled with a CsCl-based solution to voltage clamp the currents. All experiments were done in the presence of 50 microM 2-amino-5-phosphonovaleric acid (APV) and 20 microM 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) to block ionotropic glutamate-induced currents and polysynaptic transmission in the slice preparation. 2. Substituting strontium (Sr2+) for extracellular calcium (Ca2+) led to the appearance of numerous 'asynchronous' small IPSCs following an eIPSC. These asynchronous IPSCs were indistinguishable from TTX-insensitive quantal IPSCs. 3. Although somewhat less effective than Ca2+, Sr2+ was capable of supporting DSI, and both asynchronous and synchronous IPSCs were blocked by the DSI process. 4. During DSI, quantal content of eIPSCs, but not quantal size, was significantly reduced. 5. Sr2+ converted paired-pulse depression (PPD) of eIPSCs to a paired-pulse facilitation (PPF), presumably by altering the probability of release at i...Continue Reading

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Citations

Mar 30, 1999·Biophysical Journal·M A Xu-Friedman, W G Regehr
Apr 13, 2001·Neuron·J M Montgomery, D V Madison
Oct 12, 2012·Journal of Neurophysiology·Claire L De-May, Afia B Ali
Dec 17, 2009·Journal of Neurophysiology·Ping Jun Zhu, David M Lovinger
Apr 3, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Miranda A KarsonBradley E Alger
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·H W Tao, M Poo
Oct 9, 2002·Neuropharmacology·Namita VarmaBradley Alger
Dec 16, 1998·The Journal of Physiology·E Rumpel, J C Behrends
Jun 6, 2006·Brain Research Bulletin·Chi-Ming HuangRosa H Huang
May 23, 2012·The Journal of Physiology·Zheng-Quan Tang, Yong Lu

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