Src family tyrosine kinase regulates acrosome reaction but not motility in porcine spermatozoa

Reproduction : the Official Journal of the Society for the Study of Fertility
M Julia BragadoLuis J Garcia-Marin

Abstract

During the capacitation process, spermatozoa acquire the ability to fertilize an oocyte, and upregulation of cAMP-dependent protein tyrosine phosphorylation occurs. Recently, Src family tyrosine kinase (SFK) has been involved in spermatozoa capacitation as a key PKA-dependent tyrosine kinase in several species. This work investigates the expression and role of SFK in porcine spermatozoa. SFK members Lyn and Yes are identified in porcine spermatozoa by western blotting as well as two proteins named SFK1 and SFK2 were also detected by their tyrosine 416 phosphorylation, a key residue for SFK activation. Spermatozoa with SFK1 and SFK2 increase their Y416 phosphorylation time-dependently under capacitating conditions compared with noncapacitating conditions. The specific SFK inhibitor SU6656 unaffected porcine spermatozoa motility or viability. Moreover, SFK inhibition in spermatozoa under capacitating conditions leads to a twofold increase in both nonstimulated and calcium-induced acrosome reaction. Our data show that capacitating conditions lead to a time-dependent increase in actin polymerization in boar spermatozoa and that long-term incubation with SFK inhibitor causes a reduction in the F-actin content. In summary, this work ...Continue Reading

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Citations

Jul 19, 2015·General and Comparative Endocrinology·B R Lackey, S L Gray
Sep 15, 2012·Molecular Nutrition & Food Research·Francesca De AmicisSaveria Aquila
Jun 16, 2015·Theriogenology·A Hurtado de LleraM J Bragado
Mar 3, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Durgesh Kumar SinghArchana Bharadwaj Siva
Mar 14, 2020·International Journal of Molecular Sciences·Anna MańkowskaLeyland Fraser
Apr 23, 2017·Cell and Tissue Research·Deneb Maldonado-GarcíaEnrique O Hernández-González
Mar 6, 2021·Reproductive Biology and Endocrinology : RB&E·Peibei SunYa Ni

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