SRC kinase family inhibitor PP2 promotes DMSO-induced cardiac differentiation of P19 cells and inhibits proliferation

International Journal of Cardiology
Jie GongZhi-jun Jiao

Abstract

It has been reported recently that PP2, a Src family kinase inhibitor, promotes selective cardiogenesis in embryonic stem cells. However, there is no other research proved pro-cardiogenic characteristic of PP2 so far. In this study, we explored the potential cardiogenic effect of PP2 on P19 cells differentiation. P19-αMHC-EGFP cell line was established by transfecting P19 cells with αMHC-EGFP vector in order to evaluate cardiogenesis with EGFP. P19-αMHC-EGFP cells and P19 cells were induced to differentiate into cardiomyocytes with 1%DMSO, 5 μmol/L PP2, or both 1%DMSO and 5 μmol/L PP2. Differentiated cells from P19-αMHC-EGFP cells were then assessed under confocal microscope. Western-blot and RT-PCR were also performed to detect expression of cardiac troponin I and cardiac transcription factors respectively. In addition, the effects of PP2 on proliferation of P19 cells were further examined using Cell Counting Kit-8. EGFP positive cells were firstly detected on day 7 and PP2 alone cannot induce efficient cardiac differentiation of P19-αMHC-EGFP cells. However PP2 supplementation dramatically increases DMSO induced cardiac differentiation than DMSO alone. It was also found that PP2 inhibit proliferation of P19 cells in both a do...Continue Reading

References

Feb 1, 1982·Developmental Biology·M W McBurney, B J Rogers
Feb 26, 1993·Biochemical and Biophysical Research Communications·S M BloemersS W de Laat
Jul 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Joanne PaquinJolanta Gutkowska
Apr 2, 2003·Circulation·Tomosaburo TakahashiRichard T Lee
May 22, 2003·Cardiovascular Research·Marcel A G van der Heyden, Libert H K Defize
Jan 2, 2004·The Journal of Biological Chemistry·Katsuhisa MatsuuraIssei Komuro
Feb 12, 2004·Journal of the American Chemical Society·Xu WuPeter G Schultz
Jan 22, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E KolossovB K Fleischmann
Jan 25, 2005·Experimental & Molecular Medicine·Seung Cheol ChoiDo-Sun Lim
Sep 15, 2005·The Journal of Biological Chemistry·Daihiko HakunoRichard T Lee
Dec 28, 2005·Biochemical and Biophysical Research Communications·Mehdi KadivarMasoud Soleimani
Jan 18, 2006·The Journal of Physiology·Georges DaoudJulie Lafond
May 13, 2006·Circulation Research·Mohamed NemirFreddy Radtke
Jun 15, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·John C AngelloStephen D Hauschka
Jun 10, 2008·International Journal of Cardiology·Fardin FathiSeyed Javad Mowla
Nov 18, 2008·Stem Cells and Development·Frédéric Bouchard, Joanne Paquin
Dec 2, 2009·Korean Circulation Journal·Jihyun YangDo-Sun Lim
Jul 29, 2010·International Journal of Molecular Medicine·De-Liang HuLing-Mei Qian

❮ Previous
Next ❯

Citations

Dec 3, 2013·Canadian Journal of Physiology and Pharmacology·Sreejit ParameswaranRajendra K Sharma
Aug 13, 2014·Acta Biochimica Et Biophysica Sinica·Xinghua ShenXueqi Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.