Src phosphorylates the insulin-like growth factor type I receptor on the autophosphorylation sites. Requirement for transformation by src.

The Journal of Biological Chemistry
J E PetersonM J Weber

Abstract

The insulin-like growth factor type I (IGF-I) receptor can become tyrosine phosphorylated and enzymatically activated either in response to ligand or because of the activity of the Src tyrosine kinase (Peterson, J. E., Jelinek, T., Kaleko, M., Siddle, K., and Weber, M. J. (1994) J. Biol. Chem. 269, 27315-27321). The goal of the present study was to analyze the mechanistic basis and functional significance of the Src-induced phosphorylation and activation of the IGF-I receptor. 1) We mapped the sites of IGF-I receptor autophosphorylation to peptides representing three different receptor domains: tyrosines 943 and 950 in the juxtamembrane region; tyrosines 1131, 1135, and 1136 within the kinase domain; and tyrosine 1316 in the carboxyl-terminal domain. The juxtamembrane and kinase-domain peptides were phosphorylated both in vivo and in vitro. The carboxyl-terminal site, although phosphorylated in vitro and in src-transformed cells, was not a major site of ligand-induced phosphorylation in vivo. 2) We determined that the sites of Src-induced phosphorylation of the IGF-I receptor are the same as the ligand-induced autophosphorylation sites and that the Src kinase can catalyze these phosphorylations directly. 3) We showed that cells...Continue Reading

References

Apr 3, 1992·Cell·G I EvanD C Hancock
Jan 1, 1991·Methods in Enzymology·D O MorganH E Varmus
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J L TreadwayJ E Pessin
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y ZickA M Spiegel
Jun 1, 1988·Journal of Virology·M J Welham, J A Wyke
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R H AebersoldS B Kent
Jan 1, 1983·Methods in Enzymology·J A CooperT Hunter
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·O M RosenM H Cobb
Feb 1, 1994·Current Opinion in Genetics & Development·E A HarringtonG I Evan
Aug 1, 1993·Molecular Reproduction and Development·J E Pessin, A L Frattali

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Citations

Nov 26, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hazem HallakRaphael Rubin
Apr 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Geneviève Oligny-LongpréMichel Bouvier
Mar 4, 2000·Alcoholism, Clinical and Experimental Research·A E SeilerR Rubin
Jan 1, 1997·Annual Review of Cell and Developmental Biology·S M Thomas, J S Brugge
Aug 5, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·John J ArcaroliWells A Messersmith
Jul 30, 2010·PloS One·Hanna Y IrieJoan S Brugge
Apr 28, 2011·The Oncologist·Lauren N PulsWells Messersmith
Jul 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Fumitaka KogaLen Neckers
Jul 13, 2014·Journal of Dermatological Science·Aleksander HinekThomas F Mitts
Jun 18, 2010·Canadian Journal of Physiology and Pharmacology·Ali BouallegueAshok K Srivastava
Sep 17, 2014·International Journal of Molecular Sciences·Qingbin KongYangfu Jiang
Jun 11, 2010·The Journal of Investigative Dermatology·Thomas F MittsAleksander Hinek
Apr 27, 2007·The FEBS Journal·Yael GusAlexander Levitzki
Jul 4, 2006·Analytical Biochemistry·Tad GuszczynskiTerry D Copeland
Apr 26, 2011·European Journal of Pharmacology·Maria C OlianasPierluigi Onali
Mar 31, 2006·Biology of Reproduction·Robert X-D Song, Richard J Santen
Mar 14, 2007·Journal of Neurochemistry·Qiao-Ling Cui, Guillermina Almazan
Oct 19, 2004·Oncogene·Paul A BromannSara A Courtneidge
Jun 27, 1998·The Journal of Biological Chemistry·R M BaxterA Kazlauskas
Apr 5, 2000·The Journal of Biological Chemistry·A Danilkovitch-MiagkovaE J Leonard
Oct 31, 2015·Oncotarget·Md Maqusood AlamHo-Young Lee

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