PMID: 8604338Mar 15, 1996Paper

SRE elements are binding sites for the fusion protein EWS-FLI-1

Nucleic Acids Research
L Magnaghi-JaulinA Harel-Bellan

Abstract

EWS-FLI-1 is a chimeric protein produced in most Ewing's sarcomas. It results from the fusion of the N-terminal-encoding region of the EWS gene to the C-terminal DNA-binding domain (the ETS domain) encoded by the FLI-1 ets family gene. Both EWS-FLI-1 and FLI-1 proteins function as transcription factors that bind specifically to ets sequences (the ets boxes) present in promoter elements. EWS- FLI-1 is a powerful transforming protein, whereas FLI-1 is not. In a search for potential DNA binding sites for these two proteins, we have tested their ability to recognize the serum responsive element (SRE) in the c-fos promoter. This cis element contains an ets box which can be occupied by members of the ETS protein family which do not bind DNA autonomously but form a ternary complex with a second protein, p67SRF (serum responsive factor). We demonstrate here that EWS-FLI-1, but not FLI-1, is able to form a ternary complex on the c-fos SRE. Using a GST pull-down assay, we show that both FLI-1 and EWS-FLI-1 interact in vitro with SRF in the absence of DNA. In electromobility shift assays, EWS-FLI-1 binding to the SRE is detectable in the absence of SRF whereas the binding of FLI-1 is not, suggesting that the interaction with DNA is the st...Continue Reading

References

Oct 1, 1992·Trends in Biochemical Sciences·R Treisman
Jan 15, 1993·European Journal of Biochemistry·B WasylykA Giovane
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W A MayC T Denny

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Citations

Apr 14, 2005·Seminars in Cancer Biology·Heinrich Kovar
May 7, 1998·The International Journal of Biochemistry & Cell Biology·A D SharrocksP R Yates
May 3, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·S W KangM Horikoshi
Jun 19, 2001·The EMBO Journal·M Hassler, T J Richmond
Dec 22, 1999·Nucleic Acids Research·P Dalgleish, A D Sharrocks
Jul 23, 1999·Current Opinion in Oncology·H KovarA Zoubek
Oct 24, 1998·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·H HiragaK Nagashima
Jun 2, 1998·Biochimica Et Biophysica Acta·J Dittmer, A Nordheim
Aug 12, 1996·FEBS Letters·L Magnaghi-JaulinA Harel-Bellan
Apr 15, 2015·Cancer Treatment Reviews·Yunyun JiangFilip Janku
Nov 27, 1999·The Journal of Biological Chemistry·J M BosilevacS H Hinrichs
Jan 7, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·E de Alava, W L Gerald
Oct 19, 2001·Oncogene·A Arvand, C T Denny

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