Srebf1a is a key regulator of transcriptional control for adipogenesis.

Scientific Reports
Jorge-Tonatiuh Ayala-SumuanoW Kuri-Harcuch

Abstract

Adipogenesis is regulated by a complex cascade of transcriptional factors, but little is known about the early events that regulate the adipogenic program. Here, we report the role of the srebf1a gene in the differentiation of fibroblastic 3T3-F442A cells. We found that expression of srebf1a depended on GSK3β activity and that GSK3β activity was necessary for C/EBPβ phosphorylation at Thr188. Knockdown of srebf1a inhibited the adipogenic program because it blocked the expression of genes encoding PPARγ2, C/EBPα, SREBP1c and even FABP4, demonstrating that SREBP1a activation is upstream of these three essential adipogenic transcription factors. Kinetic analysis during differentiation illustrated that the order of expression of adipogenic genes was the following: cebpb, srebf1a, pparg2, cebpa, srebp1c and fabp4. Our data suggest that srebf1a acts as an essential link between the GSK3β-C/EBPβ signaling axis and the beginning of the adipogenic transcriptional cascade.

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Citations

Aug 15, 2015·Journal of Cellular Biochemistry·Jorge-Tonatiuh Ayala-SumuanoWalid Kuri-Harcuch
Jun 19, 2013·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·R J AtkinsC M Hovens
Sep 24, 2015·Biochimica Et Biophysica Acta·Claudia Hernández-MosqueiraWalid Kuri-Harcuch
Oct 10, 2015·Adipocyte·Carla Cervantes-CamachoWalid Kuri-Harcuch
Apr 8, 2014·Biochemical and Biophysical Research Communications·María Soledad AlvarezMarta Casado
Aug 15, 2013·The International Journal of Biochemistry & Cell Biology·Enrico RagniLorenza Lazzari
Feb 5, 2013·Biochemical and Biophysical Research Communications·Carlos J López-VictorioWalid Kuri-Harcuch
Dec 9, 2016·Cytotechnology·Ayşegül DoğanFikrettin Şahin
Jul 5, 2017·Journal of Agricultural and Food Chemistry·Delin MoYaosheng Chen
Nov 16, 2017·Scientific Reports·Giorgia MaroniDmitry Penkov
Aug 30, 2017·Nature Reviews. Endocrinology·Hitoshi Shimano, Ryuichiro Sato
Aug 28, 2018·Journal of Cellular Physiology·Walid Kuri-HarcuchVeronica Fernandez-Sanchez
Mar 24, 2016·Endocrinology·Jonathan G BoucherElla Atlas
Jul 29, 2020·Frontiers in Pharmacology·Srinu TumparaSabina Janciauskiene
Oct 1, 2019·Differentiation; Research in Biological Diversity·Ann-Cathrin VolzPetra Juliane Kluger
Sep 7, 2020·Differentiation; Research in Biological Diversity·Muhammad Sufyan VohraEng Hwa Wong

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