SREBP2 contributes to cisplatin resistance in ovarian cancer cells

Experimental Biology and Medicine
Lei ZhengXiu-An Yang

Abstract

This study is to investigate transcription factors involved in cisplatin resistance in ovarian cancer cells. The transcriptome of cisplatin resistant and sensitive A2780 epithelial ovarian cancer cells was obtained from GSE15372. Ovarian transcriptome data GSE62944 was downloaded from TCGA and applied for transcription regulatory network analysis. The analysis results were confirmed using quantitative polymerase chain reaction. The roles of SREBP2 in cisplatin-resistant cells were investigated by RNA inference and cell viability analysis. Transcription regulatory network analysis found that 12 transcription factors and their targets were involved in cisplatin resistant in A2780 cells. Among these factors, the targets of EZH2 and SREBP2 revealed by Transcriptional Regulatory Relationships Unraveled by Sentence-based Text mining were also enriched in differentially expressed genes between cisplatin resistant and cisplatin sensitive cells. Their targets were enriched mainly in cell cycle and cholesterol metabolic process, respectively. Bioinformatic analysis illustrated three known targets of SREBP2, namely LDLR, FDFT1, and HMGCR were increased in A2780-resistant cell lines. Additionally, the three genes and SREBP2 were also eleva...Continue Reading

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Citations

Sep 11, 2019·Anti-cancer Drugs·Yi-Shan HuangXiao-Ping Wu
Jan 29, 2020·Journal of Cellular and Molecular Medicine·Jun MaMingyi Shang
Jan 22, 2019·Frontiers in Pharmacology·Huanbiao MoHoda Yeganehjoo
Oct 6, 2020·Frontiers in Oncology·Tao JiangZhaohuan Lou
Jul 31, 2020·BMC Cancer·Andy GöbelTilman D Rachner
Nov 17, 2020·Frontiers in Oncology·Zhaodong JiGong Yang
Feb 12, 2021·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Naila ShoaibNousheen Zaidi
Mar 23, 2021·Translational Oncology·Shyamananda Singh MayengbamManoj Kumar Bhat
Sep 3, 2020·Archives of Biochemistry and Biophysics·Jiaojiao LuLe Zhao

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Datasets Mentioned

BETA
GSE15372
GSE62944

Methods Mentioned

BETA
transfection
xenograft

Software Mentioned

Graphpad
RTN package
limma
STRING
TRRUST
RTN
Prism6
TRNA
R

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