Jun 3, 1991

SRH1 protein, the yeast homologue of the 54 kDa subunit of signal recognition particle, is involved in ER translocation of secretory proteins

FEBS Letters
Y Amaya, A Nakano

Abstract

The function of the SRH1 product, the yeast homologue of the 54 kDa subunit of the mammalian signal recognition particle, has been analyzed using a galactose dependent mutant of the gene. SRH1 has been placed under control of the GAL1 promoter and introduced into a haploid cell that had its chromosomal SRH1 copy disrupted. This mutant grows normally on galactose medium but slows down the growth about 10 h after transfer to glucose medium. At the same time, precursor forms of secretory proteins, alpha-mating factor and invertase, accumulate in the cells. This result indicates that the SRH1 product is involved in translocation of precursors of secretory proteins across the endoplasmic reticulum membrane in yeast cells.

Mentioned in this Paper

Saccharomyces cerevisiae Proteins
Galactose Measurement
GAL
SRP68
Promoter
Galactose
Invertase
Haploid Cell
Sacrosidase
LGALS1 gene

About this Paper

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