SRM targeted proteomics in search for biomarkers of HCV-induced progression of fibrosis to cirrhosis in HALT-C patients.

Proteomics
Shizhen QinLeroy E Hood

Abstract

The current gold standard for diagnosis of hepatic fibrosis and cirrhosis is the traditional invasive liver biopsy. It is desirable to assess hepatic fibrosis with noninvasive means. Targeted proteomic techniques allow an unbiased assessment of proteins and might be useful to identify proteins related to hepatic fibrosis. We utilized selected reaction monitoring (SRM) targeted proteomics combined with an organ-specific blood protein strategy to identify and quantify 38 liver-specific proteins. A combination of protein C and retinol-binding protein 4 in serum gave promising preliminary results as candidate biomarkers to distinguish patients at different stages of hepatic fibrosis due to chronic infection with hepatitis C virus (HCV). Also, alpha-1-B glycoprotein, complement factor H and insulin-like growth factor binding protein acid labile subunit performed well in distinguishing patients from healthy controls.

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Citations

Feb 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Younhee KoNathan D Price
Oct 18, 2013·Science Translational Medicine·Xiao-jun LiPaul Kearney
Jan 9, 2013·Investigative Ophthalmology & Visual Science·Sanjoy K BhattacharyaUNKNOWN Seventh ARVO/Pfizer Ophthalmics Research Institute Conference Working Group
Oct 29, 2013·Annual Review of Pharmacology and Toxicology·Christopher LaustedKai Wang
Feb 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ji-Hoon ChoLeroy Hood
Dec 1, 2012·Expert Review of Proteomics·Lokesh P Tripathi, Kenji Mizuguchi
May 24, 2014·Journal of Proteome Research·Catherine PanJing Zhang
Jul 21, 2012·Biotechnology Journal·Leroy HoodCharles Auffray
May 3, 2014·The Journal of General Virology·K GouthamchandraSaumitra Das
Oct 23, 2012·Genomics, Proteomics & Bioinformatics·Leroy Hood, Qiang Tian
Jun 16, 2016·Proteomics·Tujin ShiRichard D Smith
Jan 10, 2014·Molecular & Cellular Proteomics : MCP·Erin Shammel BakerRichard D Smith
Oct 18, 2019·Nature Reviews. Clinical Oncology·James T YurkovichLeroy Hood
Nov 11, 2020·International Journal of Molecular Sciences·Ming-Ling ChangSien-Sing Yang
Oct 17, 2019·Journal of Proteome Research·Yong ZhouLeroy Hood

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