SRRF: Universal live-cell super-resolution microscopy.

The International Journal of Biochemistry & Cell Biology
Siân CulleyRicardo Henriques

Abstract

Super-resolution microscopy techniques break the diffraction limit of conventional optical microscopy to achieve resolutions approaching tens of nanometres. The major advantage of such techniques is that they provide resolutions close to those obtainable with electron microscopy while maintaining the benefits of light microscopy such as a wide palette of high specificity molecular labels, straightforward sample preparation and live-cell compatibility. Despite this, the application of super-resolution microscopy to dynamic, living samples has thus far been limited and often requires specialised, complex hardware. Here we demonstrate how a novel analytical approach, Super-Resolution Radial Fluctuations (SRRF), is able to make live-cell super-resolution microscopy accessible to a wider range of researchers. We show its applicability to live samples expressing GFP using commercial confocal as well as laser- and LED-based widefield microscopes, with the latter achieving long-term timelapse imaging with minimal photobleaching.

Citations

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Methods Mentioned

BETA
fluorescence microscopy
electron microscopy
super-resolution microscopy
fluorescence imaging
confocal microscopy
transfection
Fluorescence

Software Mentioned

NanoJ
SRRF
RESOLFT
SOFI
SQUIRREL

Related Concepts

Related Papers

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
Sébastien HerbertRicardo Henriques
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