SRST2: Rapid genomic surveillance for public health and hospital microbiology labs

BioRxiv : the Preprint Server for Biology
Michael InouyeKathryn E Holt

Abstract

Rapid molecular typing of bacterial pathogens is critical for public health epidemiology, surveillance and infection control, yet routine use of whole genome sequencing (WGS) for these purposes poses significant challenges. Here we present SRST2, a read mapping-based tool for fast and accurate detection of genes, alleles and multi-locus sequence types (MLST) from WGS data. Using >900 genomes from common pathogens, we show SRST2 is highly accurate and outperforms assembly-based methods in terms of both gene detection and allele assignment. Here we have demonstrated the use of SRST2 for microbial genome surveillance in a variety of public health and hospital settings. In the face of rising threats of antimicrobial resistance and emerging virulence amongst bacterial pathogens, SRST2 represents a powerful tool for rapidly extracting clinically useful information from raw WGS data. Source code is available from <http://katholt.github.io/srst2/>.

Related Concepts

Alleles
Antibiotics
Face
Genes
Genome
Hospitals
Hospitals, Public
Virulence
Infection Surveillance
Nucleic Acid Sequencing

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