ssDNA Aptamer Specifically Targets and Selectively Delivers Cytotoxic Drug Doxorubicin to HepG2 Cells

PloS One
Ge YuYouli Zu

Abstract

Hepatocellular carcinoma (HCC) is the third leading cause of death due to cancer worldwide with over 500,000 people affected annually. Although chemotherapy has been widely used to treat patients with HCC, alternate modalities to specifically deliver therapeutic cargos to cancer cells have been sought in recent years due to the severe side effects of chemotherapy. In this respect, aptamer-based tumor targeted drug delivery has emerged as a promising approach to increase the efficacy of chemotherapy and reduce or eliminate drug toxicity. In this study, we developed a new HepG2-specific aptamer (HCA#3) by a procedure known as systematic evolution of ligands by exponential enrichment (SELEX) and exploited its role as a targeting ligand to deliver doxorubicin (Dox) to HepG2 cells in vitro. The selected 76-base nucleotide aptamer preferentially bound to HepG2 hepatocellular carcinoma cells but not to control cells. The aptamer HCA#3 was modified with paired CG repeats at the 5'-end to carry and deliver a high payload of intercalated Dox molecules at the CG sites. Four Dox molecules (mol/mol) were fully intercalated in each conjugate aptamer-Dox (ApDC) molecule. Biostability analysis showed that the ApDC molecules are stable in serum...Continue Reading

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Citations

Oct 9, 2018·Current Medicinal Chemistry·Fen HeYanfei Liu
May 6, 2020·Drug Design, Development and Therapy·Xiao-Xia YuYin-Lin Ge
Dec 1, 2020·Cancer Biology & Therapy·Chin-Yu WangChung-Hsuan Chen
May 1, 2021·Pharmaceuticals·Frank H T NelissenHans A Heus
Oct 2, 2021·Therapeutic Delivery·Jagadish NateshMuniasamy Neerathilingam

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Methods Mentioned

BETA
surgical resection
flow cytometry
PCR
SELEX
fluorescence microscopy
fluorescence imaging
fluorescence spectroscopy
Fluorescence

Software Mentioned

Excel
integral Solver
Mfold
MS

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