SSEA-3 and 4 are not essential for the induction or properties of mouse iPS cells

Journal of Oral Science
Kazunori HamamuraKoichi Furukawa

Abstract

Stage-specific embryonic antigens (SSEA-1, 3, and 4) are carbohydrate antigens that have been used as markers of embryonic stem (ES) cells. However, the roles of these antigens in the establishment and maintenance of stemness of ES and induced pluripotent stem (iPS) cells are still poorly understood. This study investigated the biological and functional significance of globo-series glycolipids such as SSEA-3 and 4 in mouse iPS cells induced from tail-tip fibroblasts (TTFs) of α1,4Gal-T-knockout mice (lacking SSEA-3 and 4). These iPS cells were induced by retroviral transduction of four factors (Oct3/4, Sox2, Klf4, and c-Myc) into TTFs, and colonies were picked up. Morphologically, the colonies resembled ES cells and were positive for alkaline phosphatase and ES cell markers. Furthermore, in vitro-differentiated induction experiments after embryoid body formation revealed that some colonies derived from α1, 4Gal-T knockout mice were able to differentiate into three germ layers. Three germ layers were also observed in teratomas from iPS cells derived from α1,4Gal-T-knockout mice. These results suggest that SSEA-3 and 4 are not essential, at least for the establishment and maintenance of stemness of mouse iPS cells.

References

Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Senitiroh Hakomori
Apr 26, 2003·Cloning and Stem Cells·M K CarpenterM S Rao
Oct 7, 2004·Glycoconjugate Journal·Takashi Muramatsu, Hisako Muramatsu
Jul 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kazunori HamamuraKoichi Furukawa
Oct 26, 2006·Blood·Eun J GangRita C R Perlingeiro
Jun 8, 2007·Nature·Keisuke OkitaShinya Yamanaka
Dec 15, 2007·Nature Protocols·Kazutoshi TakahashiShinya Yamanaka
May 28, 2009·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jie XuZhongchao Han
Apr 12, 2013·Journal of the American Chemical Society·Ting-Chun HungChi-Huey Wong
Feb 7, 2014·The EMBO Journal·Haruhisa InoueShinya Yamanaka
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yi-Wei LouChi-Huey Wong
Apr 2, 2014·Molecular Brain·Hideyuki Okano, Shinya Yamanaka
Aug 7, 2014·Trends in Pharmacological Sciences·Ralf HeilkerJared Sterneckert
Nov 9, 2014·Journal of Biochemistry·Tamao Endo
Feb 18, 2015·Archives of Biochemistry and Biophysics·Koichi FurukawaKeiko Furukawa
Mar 12, 2019·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Koichi FurukawaKeiko Furukawa
Mar 22, 2019·Cancer Science·Koichi FurukawaKeiko Furukawa

❮ Previous
Next ❯

Citations

May 4, 2021·Frontiers in Cell and Developmental Biology·Yuange LiShen Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.