SSGF-I, a potent growth-promoting substance for mammalian cells from swine serum.

Cytotechnology
Y ShintaniK Kitano

Abstract

A small amount of swine serum markedly stimulated cell growth for high productivity subclones derived from a mouse human-human heterohybridoma, N12-16.63, secreting an anti-tetanus toxoid human monoclonal antibody in a polyethylene glycol (PEG)-containing serum-free medium, PEG-86-1. A growth promoting substance, SSGF-I, was isolated from the serum by ammonium sulfate fractionation, Cibacron blue F3A-G affinity chromatography, DEAE-agarose ion exchange chromatography, and gel filtrations on Trisacryl GF 2000 and Sephacryl S-300. SSGF-I was characterized as a low density lipoprotein (LDL) of swine serum by its physico-chemical properties. It promoted cell growth synergistically with PEG and its optimum concentration was 1 to 100μg/ml. Human LDL was less active, and human or swine high density lipoprotein (HDL) and very low density lipoprotein (VLDL) were inactive. Based on these results, we propose an improved serum-free medium, PEG-86-3, which contains all the ingredients of PEG-86-1 and 10μg/ml SSGF-I. This medium is useful for not only high productivity heterohybridomas but also for a variety of lymphoid cell lines.

References

Aug 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·M TakayamaI Tanimizu
May 31, 1985·Biochemical and Biophysical Research Communications·Y IchimoriK Tsukamoto
Dec 19, 1986·Biochimica Et Biophysica Acta·M EsfahaniM C Phillips
Jun 1, 1987·The Journal of Experimental Medicine·J D SatoT Okamoto
Jul 1, 1985·Journal of Cellular Physiology·P LibbyE J Schaefer
Aug 1, 1968·Canadian Journal of Biochemistry·M Janado, W G Martin
Mar 1, 1980·Analytical Biochemistry·D Barnes, G Sato
Oct 1, 1980·The Journal of Clinical Investigation·J P TauberD Gospodarowicz
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON

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