SSU1 Checkup, a Rapid Tool for Detecting Chromosomal Rearrangements Related to the SSU1 Promoter in Saccharomyces cerevisiae : An Ecological and Technological Study on Wine Yeast

Frontiers in Microbiology
Philippe MarulloIsabelle Masneuf-Pomarede

Abstract

Chromosomal rearrangements (CR) such as translocations, duplications and inversions play a decisive role in the adaptation of microorganisms to specific environments. In enological Saccharomyces cerevisiae strains, CR involving the promoter region of the gene SSU1 lead to a higher sulfite tolerance by enhancing the SO2 efflux. To date, three different SSU1 associated CR events have been described, including translocations XV-t-XVI and VIII-t-XVI and inversion inv-XVI. In the present study, we developed a multiplex PCR method (SSU1 checkup) that allows a rapid characterization of these three chromosomal configurations in a single experiment. Nearly 600 S. cerevisiae strains collected from fermented grape juice were genotyped by microsatellite markers. We demonstrated that alleles of the SSU1 promoter are differently distributed according to the wine environment (cellar versus vineyard) and the nature of the grape juice. Moreover, rearranged SSU1 promoters are significantly enriched among commercial starters. In addition, the analysis of nearly isogenic strains collected in wine related environments demonstrated that the inheritance of these CR shapes the genetic diversity of clonal populations. Finally, the link between the natu...Continue Reading

References

Aug 2, 2006·FEMS Yeast Research·Erlend AaJohn W Taylor
May 15, 2007·Molecular Ecology·Jean-Luc LegrasFrancis Karst
Nov 1, 2008·The Journal of Microbiology·Hoon Park, Yoon-Sun Hwang
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maite NovoSylvie Dequin
Nov 19, 2009·Applied Microbiology and Biotechnology·Naoufel CheraitiJean-Michel Salmon
Mar 8, 2011·Comptes rendus biologies·Delphine Sicard, Jean-Luc Legras
Jun 7, 2012·Applied Microbiology and Biotechnology·Benoit DivolEdward Duckitt
Jun 22, 2012·PLoS Genetics·Elizabeth K Engle, Justin C Fay
Feb 7, 2015·Genetics·Anthony R Borneman, Isak S Pretorius
May 9, 2015·Molecular Biology and Evolution·Katie J ClowersAudrey P Gasch
Jul 25, 2015·FEMS Yeast Research·Souhir Marsit, Sylvie Dequin
Apr 13, 2018·Nature·Jackson PeterJoseph Schacherer
Mar 13, 2019·Environmental Microbiology·Estéfani García-RíosJosé M Guillamón
Aug 30, 2019·PLoS Genetics·Aubin FleissGilles Fischer
Dec 14, 2019·Microbial Cell·Estéfani García-Ríos, José Manuel Guillamón

❮ Previous
Next ❯

Citations

Jun 10, 2021·Applied Microbiology and Biotechnology·Louise BartlePhilippe Marullo
Jun 6, 2021·FEMS Yeast Research·Caroline BartelAnthony Borneman

❮ Previous
Next ❯

Datasets Mentioned

BETA
2310529

Methods Mentioned

BETA
PCRs
PCR
membrane filtration
PCA
Genotyping

Software Mentioned

adegenet
R package growthrates
poppr R package
GeneMarker

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.