ST18 affects cell-cell adhesion in pemphigus vulgaris in a tumour necrosis factor-α-dependent fashion.

The British Journal of Dermatology
S AssafEli Sprecher

Abstract

Pemphigus vulgaris (PV) is a life-threatening mucocutaneous autoimmune blistering disease. We previously showed that genetic variants within the ST18 gene promoter area confer a sixfold increase in the propensity to develop PV. ST18, a transcription factor, was found to be overexpressed in the epidermis of patients with PV. In addition, it was found to promote autoantibody-mediated abnormal epidermal cell-cell adhesion and secretion of proinflammatory mediators by keratinocytes. To delineate the mechanism through which ST18 contributes to destabilization of cell-cell adhesion. We used quantitative reverse-transcriptase polymerase chain reaction, immunofluorescence microscopy, a luciferase reporter system, site-directed mutagenesis, chromatin immunoprecipitation (ChIP) and the dispase dissociation assay. The ChIP and luciferase reporter assays showed that ST18 directly binds and activates the TNF promoter. Accordingly, increased ST18 expression contributes to PV pathogenesis by destabilizing cell-cell adhesion in a tumour necrosis factor (TNF)-α-dependent fashion. In addition, dual immunofluorescence staining showed increased expression of both ST18 and TNF-α in the skin of patients with PV carrying an ST18-associated PV risk va...Continue Reading

References

Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Xiaoren TangSalomon Amar
Jan 1, 2004·Human Immunology·Ephraim GazitRon Loewenthal
Jan 18, 2005·Autoimmunity Reviews·Ephraim Gazit, Ron Loewenthal
Jul 5, 2005·Lancet·Jean-Claude Bystryn, Jennifer L Rudolph
Jul 21, 2005·The British Journal of Dermatology·J PardoJ M Fortea
Jan 13, 2006·The British Journal of Dermatology·F CaponK E Harman
Oct 27, 2006·The New England Journal of Medicine·John R Stanley, Masayuki Amagai
Nov 27, 2007·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·C BrickM Essakalli
Aug 5, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Julia YangDana T Graves
Sep 10, 2008·Journal of Clinical Immunology·Sheida ShamsBehrouz Nikbin
May 28, 2009·Journal of the European Academy of Dermatology and Venereology : JEADV·J JavorD Svecova
Nov 10, 2009·Autoimmunity Reviews·N Meyer, L Misery
May 25, 2011·Dermatologic Clinics·Animesh A Sinha
Sep 24, 2011·Autoimmunity·Sergei A Grando
Nov 7, 2015·Clinical, Cosmetic and Investigational Dermatology·Stamatis GregoriouDimitris Rigopoulos
Sep 12, 2016·Journal of the American Academy of Dermatology·Khalaf KridinReuven Bergman
May 12, 2017·Nature Reviews. Disease Primers·Michael KasperkiewiczMasayuki Amagai
Sep 8, 2018·Experimental Dermatology·Ifa EtesamiMohammad Keramatipour
Sep 20, 2018·The British Journal of Dermatology·S Y ZhangW Li
Feb 15, 2020·Cytokine·Soheil TavakolpourMaryam Daneshpazhooh
Apr 28, 2020·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Liron MalkiEli Sprecher

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Citations

May 18, 2021·The British Journal of Dermatology·T Hashimoto
Aug 27, 2021·Frontiers in Medicine·Thomas Schmitt, Jens Waschke

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