Stability and comparative transport capacity of cells, mureinoplasts, and true protoplasts of a gram-negative bacterium.

Journal of Bacteriology
I W De VoeR A MacLeod

Abstract

The outer layers of the cell envelope of a pseudomonad of marine origin were removed by washing the cells in 0.5 m NaCl followed by suspension in 0.5 m sucrose. The term mureinoplast has been suggested for the rod-shaped forms which resulted from this treatment. As previously established, these forms lacked the outer cell wall layers but still retained a rigid peptidoglycan structure. Mureinoplasts remained stable if suspended in a balanced salt solution containing 0.3 m NaCl, 0.05 m MgSO(4), and 0.01 m KCl but, unlike whole cells, lost ultraviolet (UV)-absorbing material if suspended in 0.5 m NaCl or 0.05 m MgCl(2). Sucrose added to the balanced salt solution also enhanced the loss of UV-absorbing material. Addition of lysozyme to suspensions of mureinoplasts in the balanced salt solution produced spherical forms which, by electron microscopy and the analysis of residual cell wall material, appeared to be true protoplasts. Only undamaged mureinoplasts, as judged by their capacity to fully retain alpha-aminoisobutyric acid, were capable of being converted to protoplasts. Protoplasts and undamaged mureinoplasts retained 100% transport capacity when compared to an equal number of whole cells. The Na(+) requirement for transport o...Continue Reading

References

Jun 16, 1967·Science·L A Heppel
Jan 1, 1967·Annual Review of Microbiology·M R Salton
Jan 1, 1968·Annual Review of Biochemistry·L Rothfield, A Finkelstein
Jul 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·G A ScarboroughE P Kennedy
Sep 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·C F Fox, E P Kennedy
Apr 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·H R Kaback, E R Stadtman
May 1, 1968·Journal of Bacteriology·W J Wiebe, G B Chapman
Jun 1, 1969·Journal of Bacteriology·I W De Voe, E L Oginsky
Aug 1, 1965·Canadian Journal of Microbiology·F L Buckmire, R A MacLeod
Oct 1, 1965·Journal of Dairy Science·E W Seitz, R M Hochster
Sep 1, 1957·Bacteriological Reviews·G N COHEN, J MONOD
Dec 1, 1958·Journal of Ultrastructure Research·A RYTER, E KELLENBERGER
Aug 1, 1962·Archives of Biochemistry and Biophysics·T D BROCK, G MOO-PENN
Sep 1, 1962·Bacteriological Reviews·R J BRITTEN, F T McCLURE
Apr 1, 1964·Journal of Bacteriology·W R BIBB, W R STRAUGHN
Jan 1, 1967·Annual Review of Biochemistry·R W Albers

❮ Previous
Next ❯

Citations

Jan 1, 1974·Archives of Microbiology·K Y ChoO Wyss
Dec 1, 1970·Journal of Bacteriology·C W ForsbergR A Macleod
Jan 1, 1975·Journal of Bacteriology·H M Hassan, R A MacLeod
Nov 1, 1971·Journal of Bacteriology·J Y D'Aoust, D J Kushner
Jan 1, 1976·Journal of Bacteriology·I W DeVoe
Oct 1, 1989·Research in Microbiology·G N FlatauM J Gauthier
Mar 1, 1974·Bacteriological Reviews·J W CostertonK J Cheng
May 1, 1972·Antimicrobial Agents and Chemotherapy·T J MacAlisterK J Cheng
Apr 1, 1977·Journal of Bacteriology·S M PearceT Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.