Stability and degradation of indocyanine green in plasma, aqueous solution and whole blood

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
Sonani MindtKai Nowak

Abstract

Fluorescence-based imaging has evolved into an important technology during recent years. Specifically indocyanine green (ICG) has invaded most fields of diagnostic and interventional medicine. Considering the numerous advantages of the substance like the rapid degradation and rare adverse reactions, ICG is currently the most commonly used fluorescing agent. High-performance liquid chromatography (HPLC) was used for measuring absorbance and fluorescence of ICG and its potential degradation compounds. Stability and degradation were evaluated under light exposure or in darkness at various temperatures. Under these conditions, degradation of ICG was evaluated over a period of 11 days. Additional, stability measurements of ICG were performed in EDTA whole blood samples at 37 °C incubation temperature while monitoring. Furthermore, we used mass spectrometric (MS) and nuclear magnetic resonance (NMR) analyses for the identification of supposed ICG degradation compound. Potential quenching effect of ICG was examined in aqueous and plasma solutions at concentrations ranging from 0.01-100 μg ml-1. When diluted in water and stored at 4 °C in the dark, ICG is stable for three days with only 20% of fluorescence intensity lost in this time-s...Continue Reading

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Citations

Dec 5, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Han YangJin Chang
Mar 23, 2021·International Journal of Pharmaceutics·Tianming CuiLonggang Wang
Jul 3, 2021·International Journal of Molecular Sciences·Giulia Della PelleNina Kostevšek
Aug 8, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Nitish KhuranaHamidreza Ghandehari
Jan 24, 2019·Analytical Chemistry·Kenneth S HettieFrederick T Chin
Aug 20, 2021·Annals of Medicine and Surgery·Erica SakamotoUlysses Ribeiro Junior
Jan 20, 2021·ACS Biomaterials Science & Engineering·Gao SongYuhui Yang
Sep 12, 2020·Journal of the American Chemical Society·Yaguang RenChengbo Liu
May 2, 2020·Visceral Medicine·Kai NowakAndreas Lutz Heinrich Gerken

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Methods Mentioned

BETA
fluorescence imaging
nuclear magnetic resonance
NMR
Fluorescence

Software Mentioned

MNova
GraphPadPrism
dstebpgp3s
TOPSPIN
Abacus

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