PMID: 9449777Jan 1, 1997Paper

Stability and identification of active-site residues of carboxymethylcellulases from Aspergillus niger and Cellulomonas biazotea

Folia Microbiologica
K S SiddiquiM I Rajoka

Abstract

Determination of the apparent pKa's of purified carboxymethylcellulases from Aspergillus niger and Cellulomonas biazotea at different temperatures and in the presence of dioxane indicated two side chain carboxyl groups which controlled the limiting rate in both organisms. The thermostability of both enzymes slightly decreased with increasing pH from 5 to 75 but was unaffected in the presence of 0.5 mmol/L Mn2+. The CMCase from C. biazotea had an activation energy of 35 kJ/mol and a half-life of 89 min in the presence of 8 mol/L urea at 40 degrees C. The half-life of CMCase from A. niger in 8 mol/L urea and at 37 degrees C was 125 min as determined by a 0-9 mol/L transverse urea gradient PAGE. The CMCases from A. niger and C. biazotea had the same thermostabilities in the absence of CMC although the enzyme from the former was more thermostable in the presence of the substrate. The CMCase from A. niger was also more efficient in hydrolyzing CMC than the enzyme from C. biazotea.

References

Dec 1, 1977·The Biochemical Journal·P L HurstM G Shepherd
Jan 1, 1979·Methods in Enzymology·K F Tipton, H B Dixon
Aug 1, 1992·The Biochemical Journal·M RangarajanB S Hartley
Apr 1, 1992·The Biochemical Journal·M Rangarajan, B S Hartley
Jan 1, 1990·Methods in Enzymology·V S Stoll, J S Blanchard
Jan 1, 1986·Methods in Enzymology·T E Creighton
Jun 3, 1985·European Journal of Biochemistry·A J Clarke, M Yaguchi
Feb 1, 1993·The Biochemical Journal·R MacarrónM Claeyssens
Dec 27, 1996·Annals of the New York Academy of Sciences·T Hashimoto

❮ Previous
Next ❯

Citations

Oct 13, 2006·Applied Microbiology and Biotechnology·Haq Nawaz BhattiA Jabbar
Aug 16, 2008·Applied Biochemistry and Biotechnology·Muhammad Rizwan JavedRaheela Perveen
Sep 21, 2013·Applied Biochemistry and Biotechnology·Namita BansalSanjeev Kumar Soni
Jan 26, 2017·International Journal of Biological Macromolecules·Bidyut R Mohapatra
Oct 13, 2007·Physical Review Letters·M Le Bars, P Le Gal
May 15, 2007·Canadian Journal of Microbiology·Haq Nawaz BhattiRaheela Perveen
Nov 21, 2007·Applied Biochemistry and Biotechnology·Ahmed Jawaard AfzalKhawar Sohail Siddiqui
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Shweta SachanAditi Singh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.