Stability and surface activity of lactate dehydrogenase in spray-dried trehalose

Journal of Pharmaceutical Sciences
M Adler, G Lee

Abstract

The stability of the model protein lactate dehydrogenase (LDH) during spray-drying and also on subsequent dry storage was examined. Trehalose was used as a carrier. The spray-drying temperatures Tinlet and Toutlet have a measurable effect on LDH inactivation. Low Tinlet produced the least process inactivation, but gave a high residual moisture content making the protein's storage stability poor. High Tinlet reduced residual moisture and improved storage stability, but at the cost of high process inactivation. As already found for other systems, addition of a surfactant (in this case polysorbate 80) could ameliorate process inactivation of LDH at Tinlet = 150 degreesC. Surfactant had, however, a deleterious effect on storage stability of LDH, the vital factor being the molar ratio of surfactant/protein in the dried product. By using electron spectroscopy it was shown that LDH has a 10 times higher surface concentration in the dried trehalose particles than expected for a homogeneous distribution. Surface tension measurements at the water/air interface proved that LDH is surface active, although the Gibbs equation appeared to be inapplicable. Calculations of spray-droplet formation time and drying time indicate than the extent of...Continue Reading

References

Mar 1, 1989·Journal of Pharmaceutical Sciences·P LabrudeB Chaillot
Jun 1, 1994·The Journal of Pharmacy and Pharmacology·J BroadheadC T Rhodes
Jan 1, 1997·Journal of Pharmaceutical Sciences·B C Hancock, G Zografi
May 1, 1997·Pharmaceutical Research·D P MillerH Corti
Mar 31, 1998·Journal of Pharmaceutical Sciences·Y F MaaS W Hsu
Aug 1, 1997·Pharmaceutical Development and Technology·Y F MaaC C Hsu
Jun 20, 1997·Biotechnology and Bioengineering·Y F Maa, C C Hsu

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Citations

Jun 1, 2011·Applied Microbiology and Biotechnology·Baokun LiWei Chen
Nov 28, 2007·Pharmaceutical Research·Reinhard Vehring
Feb 21, 2008·Pharmaceutical Research·Joshua D EngstromKeith P Johnston
Feb 10, 2010·Pharmaceutical Research·Mark Cornell ManningDerrick S Katayama
Apr 28, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Eero J SuihkoDavid C Apperley
Jan 28, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yu-li LoJung-hua Kuo
Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A B RichardsM Kurimoto
Sep 1, 2000·International Journal of Pharmaceutics·W Wang
Jun 4, 2010·Journal of the Royal Society, Interface·Heiko SchiffterSebastian Vonhoff
Sep 2, 2011·Chemical & Pharmaceutical Bulletin·Anita HafnerJelena Filipović-Grčić
May 29, 2012·Journal of Drug Targeting·Seung Woo ChungYoungro Byun
Apr 1, 2011·Drug Development and Industrial Pharmacy·B Erdinc, R J Neufeld
Jan 26, 2007·Journal of Pharmaceutical Sciences·Ahmad M Abdul-FattahMichael J Pikal
Feb 20, 2014·Journal of Pharmaceutical Sciences·Peter LassnerGeoffrey Lee
Jul 13, 2002·Journal of Pharmaceutical Sciences·Serena D WebbTheodore W Randolph
Jan 29, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Dieter VandenheuvelGuy Van den Mooter
Apr 12, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·William LeobandungNicholas A Peppas
Jan 1, 2008·International Journal of Pharmaceutics·Monireh JalalipourMohammadali Barghi
Dec 8, 2014·Advanced Drug Delivery Reviews·Olivier Mozziconacci, Christian Schöneich
Nov 8, 2003·The Journal of Pharmacy and Pharmacology·Yong-Hong LiaoGary P Martin
Mar 12, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Michael MauryGeoffrey Lee
Dec 7, 2007·Biophysical Journal·Vishard Ragoonanan, Alptekin Aksan
Oct 10, 2006·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Josh D EngstromKeith P Johnston
Nov 25, 2014·Biophysical Journal·Alireza AbazariMehmet Toner
Oct 14, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Alexander Mauerer, Geoffrey Lee
Sep 29, 2005·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Zhongshui YuRobert O Williams
Jan 15, 2014·International Journal of Pharmaceutics·Ankur Ajmera, Regina Scherließ
May 6, 2015·International Journal of Pharmaceutics·Joachim Schaefer, Geoffrey Lee
Apr 9, 2015·Critical Reviews in Food Science and Nutrition·Dianawati DianawatiNagendra P Shah
Aug 16, 2016·International Journal of Pharmaceutics·Gaurav KanojiaJean-Pierre Amorij
Mar 10, 2017·Drug Development and Industrial Pharmacy·Vahid RamezaniHamed Fanaei
Oct 17, 2018·Mini Reviews in Medicinal Chemistry·Maobin XieYi Li
Dec 25, 2019·Pharmaceutical Research·Nathan E WilsonQi Tony Zhou
Dec 25, 2019·Pharmaceutical Research·Dominique N PricePavan Muttil
Jul 9, 2008·Journal of Microencapsulation·Chirasak KusonwiriyawongGarnpimol C Ritthidej

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