Stability and tribological performances of fluid phospholipid bilayers: effect of buffer and ions

Colloids and Surfaces. B, Biointerfaces
F DekkicheJ-P Rieu

Abstract

We have investigated the mechanical and tribological properties of supported Dioleoyl phosphatidylcholine (DOPC) bilayers in different solutions: ultrapure water (pH 5.5), saline solution (150 mM NaCl, pH 5.8), Tris buffer (pH 7.2) and Tris saline buffer (150 mM NaCl, pH 7.2). Friction forces are measured using a homemade biotribometer. Lipid bilayer degradation is controlled in situ during friction tests using fluorescence microscopy. Mechanical resistance to indentation is measured by force spectroscopy with an atomic force microscope. This study confirms that mechanical stability under shear or normal load is essential to obtain low and constant friction coefficients. In ultrapure water, bilayers are not resistant and have poor lubricant properties. On the other hand, in Tris saline buffer, they fully resist to indentation and exhibit low (micro=0.035) and stable friction coefficient with no visible wear during the 50 min of the friction test. The unbuffered saline solution improves the mechanical resistance to indentation but not the lubrication. These results suggest that the adsorption of ions to the zwiterrionic bilayers has different effects on the mechanical and tribological properties of bilayers: higher resistance to...Continue Reading

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Citations

Apr 30, 2013·Biomaterials·Raya SorkinJacob Klein
Sep 23, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Aurora Dols-PerezGabriel Gomila
Apr 9, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Raya SorkinJacob Klein
May 28, 2013·Mathematical Biosciences·Adam GadomskiZenon Pawlak
Jan 3, 2015·Biointerphases·Zenon PawlakWojciech Hagner
Apr 19, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Anastasia Gaisinskaya-KipnisJacob Klein
May 3, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Zhenyu J ZhangGraham J Leggett
Apr 19, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Dmitri D Iarikov, William A Ducker
Feb 8, 2013·International Journal of Molecular Sciences·Simon J AttwoodZoya Leonenko
Mar 13, 2018·Lubricants·Teresa VeselackMarkus A Wimmer
May 16, 2020·ACS Nano·Weifeng LinJacob Klein

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