Stability, folding, dimerization, and assembly properties of the yeast prion Ure2p

Biochemistry
C ThualRonald Melki

Abstract

The [URE3] factor of Saccharomyces cerevisiae propagates by a prion-like mechanism and corresponds to the loss of the function of the cellular protein Ure2. The molecular basis of the propagation of this phenotype is unknown. We recently expressed Ure2p in Escherichia coli and demonstrated that the N-terminal region of the protein is flexible and unstructured, while its C-terminal region is compactly folded. Ure2p oligomerizes in solution to form mainly dimers that assemble into fibrils [Thual et al. (1999) J. Biol. Chem. 274, 13666-13674]. To determine the role played by each domain of Ure2p in the overall properties of the protein, specifically, its stability, conformation, and capacity to assemble into fibrils, we have further analyzed the properties of Ure2p N- and C-terminal regions. We show here that Ure2p dimerizes through its C-terminal region. We also show that the N-terminal region is essential for directing the assembly of the protein into a particular pathway that yields amyloid fibrils. A full-length Ure2p variant that possesses an additional tryptophan residue in its N-terminal moiety was generated to follow conformational changes affecting this domain. Comparison of the overall conformation, folding, and unfoldin...Continue Reading

Citations

Aug 2, 2011·Journal of Biomolecular NMR·Birgit HabensteinAnja Böckmann
May 12, 2004·Biochimica Et Biophysica Acta·Vladimir N Uversky, Anthony L Fink
Nov 1, 2003·Biochemical and Biophysical Research Communications·Li ZhuSarah Perrett
Apr 5, 2002·Microbes and Infection·Luc Bousset, Ronald Melki
Feb 28, 2002·Comptes rendus biologies·Luc BoussetRonald Melki
Jan 15, 2011·The Journal of Biological Chemistry·Yi-Qian WangSarah Perrett
Apr 11, 2008·The Journal of Biological Chemistry·Jimmy SavistchenkoRonald Melki
Jul 11, 2009·Protein Engineering, Design & Selection : PEDS·Helen M Saunders, Stephen P Bottomley
Mar 27, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Li-Qiong XuSarah Perrett
Jun 15, 2011·Molecular and Cellular Biology·Emily T CrowLiming Li
Oct 7, 2005·Biological Chemistry·Silvia CatharinoStefan Walter
Jan 10, 2002·Journal of Molecular Biology·Despina GalaniSarah Perrett
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ulrich BaxaReed B Wickner
May 15, 2004·Proceedings of the National Academy of Sciences of the United States of America·Andrey V KajavaAlasdair C Steven
Sep 11, 2001·Biochemical and Biophysical Research Communications·J M ZhouS Perrett
Jul 9, 2010·New Biotechnology·Claire NoirotAnja Böckmann
Dec 14, 2011·International Journal of Evolutionary Biology·Anne ThuillierMélanie Morel
Jul 11, 2006·Protein Science : a Publication of the Protein Society·Nuria Sánchez-Puig, Alan R Fersht
Sep 27, 2006·Protein Science : a Publication of the Protein Society·Neil RansonLouise C Serpell
Jan 24, 2006·Biochimica Et Biophysica Acta·Hui-Yong LianSarah Perrett
Nov 26, 2010·The Biochemical Journal·Yong YuSarah Perrett
Sep 23, 2011·Biochemical Society Transactions·Li-Jun ChenSarah Perrett
Oct 11, 2008·Journal of Molecular Biology·Zai-Rong ZhangSarah Perrett
Jul 27, 2002·Annual Review of Microbiology·Susan M Uptain, Susan Lindquist
Nov 20, 2003·Nature Reviews. Molecular Cell Biology·Mick F Tuite, Brian S Cox
Mar 31, 2006·The Journal of Biological Chemistry·Laura PieriMassimo Stefani
Jun 16, 2001·The Journal of Biological Chemistry·A A KulkarniT G Cooper
Jun 5, 2003·The Journal of Biological Chemistry·Nicolas FayRonald Melki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.