Stability indicating reverse phase high performance liquid chromatography method for the estimation of capsaicin

Pharmaceutical Methods
Alankar Shrivastava, Prachi Saxena


Stability Indicating Method (SIM) is a quantitative analytical procedure used to detect a decrease in the amount of the active pharmaceutical ingredient (API) present due to degradation. According to Food and Drug Administration (FDA) guidelines, an SIM is defined as a validated analytical procedure that accurately and precisely measures active ingredients (drug substance or drug product) free from potential interferences, such as degradation products, process impurities, excipients, or other potential impurities, and the FDA recommends that all assay procedures for stability studies be stability indicating. Here in this study we developed simple precise and accurate stability indicating reverse-phase high-performance liquid chromatographic (RP-HPLC) assay to analyze capsaicin (CAP) at concentrations from 70 to 130 μg/mL. HPLC equipped with Photo diode Array (PDA) detector, Pump model 600E of Waters and Empower software. Column used for the separation is ODS (250 × 4.6 mm) with particle size 5 μm and all the reagents and water were of HPLC grade. The chromatographic separation was carried out using mobile phase Acetonitrile (ACN):water:buffer::75:10:15 with a flow rate of 1 mL/min on a C18 column. The concentration of the eluti...Continue Reading


Dec 6, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·J Lu, M Cwik
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Feb 1, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria de Lourdes Reyes-EscogidoErika Vazquez-Tzompantzi

Related Concepts

Pharmacologic Substance
High Pressure Liquid Chromatography Procedure
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