PMID: 6161228Sep 1, 1980Paper

Stability of bovine adrenal medulla cells in culture

Journal of Neurochemistry
D L KilpatrickN Kirshner

Abstract

The functional stability of primary cultures of adrenal medulla cells was investigated. Isolated cells were prepared by treatment of bovine adrenal glands with collagenase followed by purification on Percoll density gradients and were maintained in Dulbecco's medium containing 10% fetal calf serum. Within 12 h after plating on plastic culture dishes, the cells became firmly attached and exhibited good survival for periods of time up to 3 weeks, as indicated by their morphology using light and electron microscopy, by maintenance of their content of catecholamines, tyrosine hydroxylase, dopamine-beta-hydroxylase, and phenylethanolamine N-methyltransferase, and their ability to respond to secretagogues. During the first 10 days to 2 weeks in culture there was little or no change in any of these parameters. During the 3rd week there were progressive losses of catecholamine and enzyme activities and increased vacuolization of medullary cells. The cells synthesized protein and RNA with no apparent loss in activities over the period studied, but did not incorporate [3H]thymidine into PCA-precipitable material. The cells responded to secretagogues and secretory antagonists similarly to isolated perfused adrenal glands. The studies desc...Continue Reading

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Citations

Sep 1, 1982·Naunyn-Schmiedeberg's Archives of Pharmacology·G B PicottiJ Meldolesi
Dec 1, 1992·Neurochemical Research·J S AguilarM Criado
Jan 12, 1988·European Journal of Pharmacology·P D MarleyB G Livett
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Feb 21, 1992·Brain Research. Developmental Brain Research·K Unsicker, R Westermann
Jan 1, 1985·Neurochemistry International·M T Miras-PortugalD Aunis

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