Stability of Bovine viral diarrhea virus 1 nucleic acid in fetal bovine samples stored under different conditions

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Julia F RidpathJill Waldbillig

Abstract

Infection of pregnant cattle with both species of Bovine viral diarrhea virus (BVDV) can result in reproductive disease that includes fetal reabsorption, mummification, abortion, stillbirths, congenital defects affecting structural, neural, reproductive, and immune systems, and the birth of calves persistently infected with BVDV. Accurate diagnosis of BVDV-associated reproductive disease is important to control BVDV at the production unit level and assessment of the cost of BVDV infections in support of BVDV control programs. The purpose of the current study was to examine the stability of viral nucleic acid in fetal tissues exposed to different conditions, as measured by detection by polymerase chain reaction. Five different types of fetal tissue, including brain, skin and muscle, ear, and 2 different pooled organ samples, were subjected to conditions that mimicked those that might exist for samples collected after abortions in production settings or possible storage conditions after collection and prior to testing. In addition, tissues were archived for 36 months at -20°C and then retested, to mimic conditions that might occur in the case of retrospective surveillance studies. Brain tissue showed the highest stability under t...Continue Reading

References

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Citations

May 18, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Maximiliano J SpetterErika A González Altamiranda

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
reverse transcription PCR
electrophoresis

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