Abstract
An increasing number of studies using primary human bronchial epithelial cells (BECs) have reported intrinsic differences in the expression of several genes between cells from asthmatic and non-asthmatic donors. The stability of gene expression by primary BECs with increasing cell passage number has not been well characterized. To determine if expression by primary BECs from asthmatic and non-asthmatic children of selected genes associated with airway remodeling, innate immune response, immunomodulatory factors, and markers of differentiated airway epithelium, are stable over increasing cell passage number, we studied gene expression patterns in passages 1, 2, 3, 4, and 5 BECs from asthmatic (n = 6) and healthy (n = 6) subjects that were differentiated at an air-liquid interface. RNA was harvested from BECs and RT-PCR was performed for TGFβ1, TGFβ2, activin A, FSTL3, MUC5AC, TSLP, IL-33, CXCL10, IFIH1, p63, KT5, TUBB4A, TJP1, OCLN, and FOXJ1. Expression of TGFβ1, TGFβ2, activin A, FSTL3, MUC5AC, CXCL10, IFIH1, p63, KT5, TUBB4A, TJP1, OCLN, and FOXJ1 by primary BECs from asthmatic and healthy children was stable with no significant differences between passages 1, 2 and 3; however, gene expression at cell passages 4 and 5 was sig...Continue Reading
References
May 9, 2001·Nucleic Acids Research·M W Pfaffl
Sep 11, 2002·Molecular Biology of the Cell·Carolyn B CoyneLarry G Johnson
Sep 6, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G M DohertyL G Heaney
Mar 17, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tadashi KikuchiDaniel J Tschumperlin
Jun 10, 2005·Respiratory Research·Catherine LaneStephen Stick
Sep 30, 2006·American Journal of Respiratory Cell and Molecular Biology·Pierre LeSimpleEdith Puchelle
Dec 13, 2006·American Journal of Respiratory and Critical Care Medicine·Stefan PierrouRatko Djukanovic
Jul 26, 2008·Stem Cells·Tillie-Louise HackettDarryl A Knight
Oct 26, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Alejandro A PezzuloJoseph Zabner
Feb 2, 2011·The Journal of Allergy and Clinical Immunology·Jan LötvallPaul A Greenberger
Jun 7, 2011·American Journal of Respiratory Cell and Molecular Biology·Tillie-Louise HackettDarryl A Knight
Jun 21, 2011·Immunological Reviews·Stephen T Holgate
Sep 3, 2011·American Journal of Respiratory and Critical Care Medicine·Raed A DweikUNKNOWN American Thoracic Society Committee on Interpretation of Exhaled Nitric Oxide Levels (FENO) for Clinical Applications
Jul 11, 2013·American Journal of Respiratory Cell and Molecular Biology·Stephanie M B WarnerDarryl A Knight
Dec 5, 2013·Respiratory Research·Matthew S WaltersRonald G Crystal
Jun 7, 2014·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·Helen K ReddelJ Mark FitzGerald
Sep 23, 2014·Nature Reviews. Immunology·Michael J HoltzmanXinyu Wang
Jul 17, 2015·Pediatric Pulmonology·Kirsty McLellanSteve Turner
Feb 4, 2016·American Journal of Respiratory and Critical Care Medicine·Colin R ButlerSam M Janes
Jun 1, 2016·International Archives of Allergy and Immunology·Dick PapazianSoren W K Hansen
May 5, 2017·BMC Pulmonary Medicine·Delphine GrasIsabelle Vachier
Citations
Mar 1, 2019·SLAS Discovery·Tijmen H BooijErik H J Danen
May 18, 2019·The European Respiratory Journal·Christine H T BuiMichael C W Chan
May 19, 2020·Annals of the American Thoracic Society·Jason S DebleyDavid P Nichols
Mar 27, 2020·Scientific Reports·Yoshikazu AraiJun Ohgane
Jul 17, 2020·Physiological Research·J HandlT Roušar
Jun 6, 2021·Scientific Reports·Mahmood Yaseen HachimRifat Hamoudi
Sep 8, 2021·Frontiers in Immunology·Laurine C RijsbergenRik L de Swart
Dec 4, 2021·The European Respiratory Journal·Stephen R Reeves
Aug 21, 2021·The European Respiratory Journal·Giovanni SetteMarco Lucarelli