PMID: 37930Jun 1, 1979

Stability of glucose-6-phosphate dehydrogenase in human cells cultivated in vitro

Biokhimii︠a︡
S B AlekseevA N Avakova

Abstract

It was shown that the thermal stability of glucose-6-phosphate dehydrogenase in human diploid cells is much higher than in human heteroploid cell lines HeLa and T-9. The purified enzymes from human diploid cells and from HeLa and T-9 cells possess similar thermal stabilities. Mixing of T-9 extracts with the purified enzyme preparations revealed that the non-stability factors of the dehydrogenase are present in the T-9 extracts. An addition of NADP- and NADPH-containing buffers and crystalline NADP to the heteroploid cell extracts stabilizes the enzyme. The thermal stability of the enzyme from "in vitro" cultivated human cells depends on the concentration of the coenzyme. It was also demonstrated that glucose-6-phosphate dehydrogenase stability in HeLa and T-9 extracts is the same at low concentrations of the coenzyme and after addition of crystalline NADP. However, at NADP concentration of 10(-3) M the enzyme stability in HeLa and T-9 extracts is different. It is assumed that the destabilizing factors are the enzymes possessing the nucleotidases activity, which is different in various cell lines.

Related Concepts

Buffers
Nucleotidases
Enzymes, antithrombotic
G6PD
Glucosephosphate Dehydrogenase
Glucose-6-phosphate Dehydrogenase Measurement, Quantitative
Ampholytes
Enzyme agent
Enzymes for Treatment of Wounds and Ulcers
Enzyme preparations, digestives

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