Stability of important antibodies for kidney disease: pre-analytic methodological considerations

PeerJ
Qiuxia HanHanyu Zhu

Abstract

The importance of circulating antibodies as biomarkers of kidney disease has recently been recognized. However, no study has systematically described the methodology of sample preparation and storage regarding antibodies as biomarkers of kidney disease. It remains unknown whether repetitive freeze-thaw cycles, physical disturbances, storage at different temperatures or for different periods of time, or haemolytic or turbid serum samples affect antibody measurements. The aim of this study was to investigate the stabilities of antibodies associated with kidney disease in serum samples under various relevant clinical and research conditions. We stored serum samples in the following different conditions: repetitive freeze-thaw cycles (1, 6 or 12 times), long-term storage (7 or 12 months at -80 °C), physical disturbance (1 or 8 h), and storage at 4 °C (1, 3 or 6 weeks) and room temperature (1 or 7 days). The stabilities of the anti-phospholipase A2 receptor (anti-PLA2R), anti-glomerular basement membrane, anti-myeloperoxidase and anti-proteinase 3 antibodies were evaluated with enzyme-linked immunosorbent assays (ELISA). We found that repetitive freeze-thaw cycles did not have a significant effect on the stabilities of the abovement...Continue Reading

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Citations

Mar 7, 2021·International Journal of Environmental Research and Public Health·Ravit BassalLital Keinan-Boker

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Methods Mentioned

BETA
as
ELISA

Software Mentioned

SPSS

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